Two sublines of a lymphoblastoid cell line associated with Marek's disease virus

Masao Yamada, H. Matsuda, S. Nii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

From MDCC-MSB1, the original Marek's disease virus-associated lymphoblastoid cell line, two sublines named MDCC-MSB1-33C and MDCC-MSB1-41C were differentiated. The MDCC-MSB1-33C cell line grew well at 33°, but grew poorly and finally stopped growing at 41° . On the other hand, the MDCC-MSB1-41C cell line grew actively at 41°, but at 33°, continuous cultivation was difficult to achieve. MDCC-MSB1-33C grew suspended in the liquid medium, as did the original producer MDCC-MSB1 cell line, while MDCC-MSB1-41C grew attached to the substrate. Moreover, in soft agar, MDCC-MSB1-33C formed loose colonies, but MDCC-MSB1-41C formed packed colonies. Neither Marek's disease viral antigens nor herpes-type particles could be detected in either subline, although a Marek's disease tumor-associated surface antigen was found on the surface of both.

Original languageEnglish
Pages (from-to)502-508
Number of pages7
JournalGann, The Japanese Journal of Cancer Research
Volume74
Issue number4
Publication statusPublished - 1983

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Marek Disease
Viruses
Cell Line
Viral Antigens
Surface Antigens
Agar
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

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Two sublines of a lymphoblastoid cell line associated with Marek's disease virus. / Yamada, Masao; Matsuda, H.; Nii, S.

In: Gann, The Japanese Journal of Cancer Research, Vol. 74, No. 4, 1983, p. 502-508.

Research output: Contribution to journalArticle

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abstract = "From MDCC-MSB1, the original Marek's disease virus-associated lymphoblastoid cell line, two sublines named MDCC-MSB1-33C and MDCC-MSB1-41C were differentiated. The MDCC-MSB1-33C cell line grew well at 33°, but grew poorly and finally stopped growing at 41° . On the other hand, the MDCC-MSB1-41C cell line grew actively at 41°, but at 33°, continuous cultivation was difficult to achieve. MDCC-MSB1-33C grew suspended in the liquid medium, as did the original producer MDCC-MSB1 cell line, while MDCC-MSB1-41C grew attached to the substrate. Moreover, in soft agar, MDCC-MSB1-33C formed loose colonies, but MDCC-MSB1-41C formed packed colonies. Neither Marek's disease viral antigens nor herpes-type particles could be detected in either subline, although a Marek's disease tumor-associated surface antigen was found on the surface of both.",
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