Two-step differentiation of mast cells from induced pluripotent stem cells

Tomoko Yamaguchi, Katsuhisa Tashiro, Satoshi Tanaka, Sumie Katayama, Waka Ishida, Ken Fukuda, Atsuki Fukushima, Ryoko Araki, Masumi Abe, Hiroyuki Mizuguchi, Kenji Kawabata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mast cells play important roles in the pathogenesis of allergic diseases. They are generally classified into 2 phenotypically distinct populations: connective tissue-type mast cells (CTMCs) and mucosal-type mast cells (MMCs). The number of mast cells that can be obtained from tissues is limited, making it difficult to study the function of mast cells. Here, we report the generation and characterization of CTMC-like mast cells derived from mouse induced pluripotent stem (iPS) cells. iPS cell-derived mast cells (iPSMCs) were generated by the OP9 coculture method or embryoid body formation method. The number of Safranin O-positive cells, expression levels of CD81 protein and histidine decarboxylase mRNA, and protease activities were elevated in the iPSMCs differentiated by both methods as compared with those in bone marrow-derived mast cells (BMMCs). Electron microscopic analysis revealed that iPSMCs contained more granules than BMMCs. Degranulation was induced in iPSMCs after stimulation with cationic secretagogues or vancomycin. In addition, iPSMCs had the ability to respond to stimulation with the IgE/antigen complex in vitro and in vivo. Moreover, when iPSMCs generated on OP9 cells were cocultured with Swiss 3T3 fibroblasts, protease activities as maturation index were more elevated, demonstrating that mature mast cells were differentiated from iPS cells. iPSMCs can be used as an in vitro model of CTMCs to investigate their functions.

Original languageEnglish
Pages (from-to)726-734
Number of pages9
JournalStem Cells and Development
Volume22
Issue number5
DOIs
Publication statusPublished - Mar 1 2013

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Induced Pluripotent Stem Cells
Mast Cells
Connective Tissue Cells
Peptide Hydrolases
Bone Marrow
Histidine Decarboxylase
Embryoid Bodies

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Yamaguchi, T., Tashiro, K., Tanaka, S., Katayama, S., Ishida, W., Fukuda, K., ... Kawabata, K. (2013). Two-step differentiation of mast cells from induced pluripotent stem cells. Stem Cells and Development, 22(5), 726-734. https://doi.org/10.1089/scd.2012.0339

Two-step differentiation of mast cells from induced pluripotent stem cells. / Yamaguchi, Tomoko; Tashiro, Katsuhisa; Tanaka, Satoshi; Katayama, Sumie; Ishida, Waka; Fukuda, Ken; Fukushima, Atsuki; Araki, Ryoko; Abe, Masumi; Mizuguchi, Hiroyuki; Kawabata, Kenji.

In: Stem Cells and Development, Vol. 22, No. 5, 01.03.2013, p. 726-734.

Research output: Contribution to journalArticle

Yamaguchi, T, Tashiro, K, Tanaka, S, Katayama, S, Ishida, W, Fukuda, K, Fukushima, A, Araki, R, Abe, M, Mizuguchi, H & Kawabata, K 2013, 'Two-step differentiation of mast cells from induced pluripotent stem cells', Stem Cells and Development, vol. 22, no. 5, pp. 726-734. https://doi.org/10.1089/scd.2012.0339
Yamaguchi T, Tashiro K, Tanaka S, Katayama S, Ishida W, Fukuda K et al. Two-step differentiation of mast cells from induced pluripotent stem cells. Stem Cells and Development. 2013 Mar 1;22(5):726-734. https://doi.org/10.1089/scd.2012.0339
Yamaguchi, Tomoko ; Tashiro, Katsuhisa ; Tanaka, Satoshi ; Katayama, Sumie ; Ishida, Waka ; Fukuda, Ken ; Fukushima, Atsuki ; Araki, Ryoko ; Abe, Masumi ; Mizuguchi, Hiroyuki ; Kawabata, Kenji. / Two-step differentiation of mast cells from induced pluripotent stem cells. In: Stem Cells and Development. 2013 ; Vol. 22, No. 5. pp. 726-734.
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