Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region

Toshio Kodama, Kazuyoshi Goto, Hirotaka Hiyoshi, Mikiharu Morita, Kaori Izutsu, Yukihiro Akeda, Kwon Sam Park, Vlademir V. Cantarelli, Rikard Dryselius, Tetsuya Iida, Takeshi Honda

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is an important pathogen causing food-borne disease worldwide. An 80-kb pathogenicity island (Vp-PAI), which contains two tdh (thermostable direct hemolysin) genes and a set of genes for the type III secretion system (T3SS2), is closely related to the pathogenicity of this bacterium. However, the regulatory mechanisms of Vp-PAI's gene expression are poorly understood. Here we report that two novel ToxR-like transcriptional regulatory proteins (VtrA and VtrB) regulate the expression of the genes encoded within the Vp-PAI region, including those for TDH and T3SS2-related proteins. Expression of vtrB was under control of the VtrA, as vector-expressed vtrB was able to recover a functional protein secretory capacity for T3SS2, independent of VtrA. Moreover, these regulatory proteins were essential for T3SS2-dependent biological activities, such as in vitro cytotoxicity and in vivo enterotoxicity. Enterotoxic activities of vtrA and/or vtrB deletion strains derived from the wild-type strain were almost absent, showing fluid accumulation similar to non-infected control. Whole genome transcriptional profiling of vtrA or vtrB deletion strains revealed that the expression levels of over 60 genes were downregulated significantly in these deletion mutant strains and that such genes were almost exclusively located in the Vp-PAI region. These results strongly suggest that VtrA and VtrB are master regulators for virulence gene expression in the Vp-PAI and play critical roles in the pathogenicity of this bacterium.

Original languageEnglish
Article numbere8678
JournalPLoS One
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 13 2010
Externally publishedYes

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Polyamideimides
Vibrio parahaemolyticus
Genes
Virulence
Gene Expression
gene expression
regulatory proteins
Proteins
pathogenicity
genes
pathogenicity islands
Bacteria
Genomic Islands
type III secretion system
Gene expression
Foodborne Diseases
hemolysins
bacteria
Regulator Genes
foodborne illness

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. / Kodama, Toshio; Goto, Kazuyoshi; Hiyoshi, Hirotaka; Morita, Mikiharu; Izutsu, Kaori; Akeda, Yukihiro; Park, Kwon Sam; Cantarelli, Vlademir V.; Dryselius, Rikard; Iida, Tetsuya; Honda, Takeshi.

In: PLoS One, Vol. 5, No. 1, e8678, 13.01.2010.

Research output: Contribution to journalArticle

Kodama, T, Goto, K, Hiyoshi, H, Morita, M, Izutsu, K, Akeda, Y, Park, KS, Cantarelli, VV, Dryselius, R, Iida, T & Honda, T 2010, 'Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region', PLoS One, vol. 5, no. 1, e8678. https://doi.org/10.1371/journal.pone.0008678
Kodama, Toshio ; Goto, Kazuyoshi ; Hiyoshi, Hirotaka ; Morita, Mikiharu ; Izutsu, Kaori ; Akeda, Yukihiro ; Park, Kwon Sam ; Cantarelli, Vlademir V. ; Dryselius, Rikard ; Iida, Tetsuya ; Honda, Takeshi. / Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. In: PLoS One. 2010 ; Vol. 5, No. 1.
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