Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

Kyoichi Azuma, Masaki Kobayashi, Masahisa Nakamura, Nobuo Suzuki, Sayaka Yashima, Shawichi Iwamuro, Mika Ikegame, Toshio Yamamoto, Atsuhiko Hattori

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37 Citations (Scopus)

Abstract

Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale.

Original languageEnglish
Pages (from-to)594-600
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number3
DOIs
Publication statusPublished - Oct 26 2007

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Keywords

  • Bone
  • Cathepsin K
  • Goldfish
  • Morphology
  • Osteoclasts
  • Scale
  • Sequence
  • Tartrate-resistant acid phosphatase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Azuma, K., Kobayashi, M., Nakamura, M., Suzuki, N., Yashima, S., Iwamuro, S., Ikegame, M., Yamamoto, T., & Hattori, A. (2007). Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales. Biochemical and Biophysical Research Communications, 362(3), 594-600. https://doi.org/10.1016/j.bbrc.2007.08.010