Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella

Kazuhiro Kutsukake, Tadayoshi Ikebe, Shouji Yamamoto

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)


The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalGenes and Genetic Systems
Issue number6
Publication statusPublished - Dec 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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