Abstract
The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.
| Original language | English |
|---|---|
| Pages (from-to) | 287-292 |
| Number of pages | 6 |
| Journal | Genes and Genetic Systems |
| Volume | 74 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Genetics
- Genetics(clinical)
Cite this
Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella. / Kutsukake, Kazuhiro; Ikebe, Tadayoshi; Yamamoto, Shouji.
In: Genes and Genetic Systems, Vol. 74, No. 6, 12.1999, p. 287-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella
AU - Kutsukake, Kazuhiro
AU - Ikebe, Tadayoshi
AU - Yamamoto, Shouji
PY - 1999/12
Y1 - 1999/12
N2 - The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.
AB - The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033497962&partnerID=8YFLogxK
U2 - 10.1266/ggs.74.287
DO - 10.1266/ggs.74.287
M3 - Article
C2 - 10791024
AN - SCOPUS:0033497962
VL - 74
SP - 287
EP - 292
JO - Genes and Genetic Systems
JF - Genes and Genetic Systems
SN - 1341-7568
IS - 6
ER -