Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella

Kazuhiro Kutsukake, Tadayoshi Ikebe, Shouji Yamamoto

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalGenes and Genetic Systems
Volume74
Issue number6
DOIs
Publication statusPublished - Dec 1999
Externally publishedYes

Fingerprint

Regulon
regulon
Salmonella
Regulator Genes
regulator genes
Genes
operon
Operon
mutation
genes
Mutation
Transcription
transcription (genetics)
sigma factors
Sigma Factor
Chromosomes
plasmids
Plasmids
promoter regions
chromosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Genetics(clinical)

Cite this

Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella. / Kutsukake, Kazuhiro; Ikebe, Tadayoshi; Yamamoto, Shouji.

In: Genes and Genetic Systems, Vol. 74, No. 6, 12.1999, p. 287-292.

Research output: Contribution to journalArticle

Kutsukake, Kazuhiro ; Ikebe, Tadayoshi ; Yamamoto, Shouji. / Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella. In: Genes and Genetic Systems. 1999 ; Vol. 74, No. 6. pp. 287-292.
@article{32f3c490fc264fb9af87d57bb0473c01,
title = "Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella",
abstract = "The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.",
author = "Kazuhiro Kutsukake and Tadayoshi Ikebe and Shouji Yamamoto",
year = "1999",
month = "12",
doi = "10.1266/ggs.74.287",
language = "English",
volume = "74",
pages = "287--292",
journal = "Genes and Genetic Systems",
issn = "1341-7568",
publisher = "Genetics Society of Japan",
number = "6",

}

TY - JOUR

T1 - Two novel regulatory genes, fliT and fliZ, in the flagellar regulon of Salmonella

AU - Kutsukake, Kazuhiro

AU - Ikebe, Tadayoshi

AU - Yamamoto, Shouji

PY - 1999/12

Y1 - 1999/12

N2 - The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.

AB - The flagellar operons of Salmonella are divided into three classes with respect to their transcriptional hierarchy. Expression of the class 2 operons requires the class 1 gene products, FlhD and FlhC, and is increased by mutation in the flgM gene, which encodes a class 3-specific anti-sigma factor. Here we report the identification of two novel regulatory genes for class 2 transcription. Presence of the fliZ and fliT genes on multicopy plasmids enhanced and inhibited, respectively, transcription from a chromosomal class 2 promoter. Disruption of the fliZ and fliT genes on the chromosome decreased and increased, respectively, class 2 expression. These results suggest that the fliZ and fliT genes may encode positive and negative regulatory factors, respectively, for class 2 expression. Enhancement of class 2 expression by the flgM mutation was cancelled by the coexisting fliZ mutation, indicating that FliZ is essential for this enhancement.

UR - http://www.scopus.com/inward/record.url?scp=0033497962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033497962&partnerID=8YFLogxK

U2 - 10.1266/ggs.74.287

DO - 10.1266/ggs.74.287

M3 - Article

VL - 74

SP - 287

EP - 292

JO - Genes and Genetic Systems

JF - Genes and Genetic Systems

SN - 1341-7568

IS - 6

ER -