Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes)

Yu Hsin Lin, Miki Fujita, Sotaro Chiba, Kiwamu Hyoudou, Andika Ida Bagus, Nobuhiro Suzuki, Hideki Kondo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

There is still limited information on the diversity of (−)ssRNA viruses that infect fungi. Here, we have discovered two novel (−)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (−)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (−)ssRNA viruses in fungi.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalVirology
Volume533
DOIs
Publication statusPublished - Jul 1 2019

Fingerprint

Shiitake Mushrooms
RNA Viruses
Viruses
Fungi
RNA
Mononegavirales
Genome
Nucleocapsid
Arthropods
Ticks

Keywords

  • Ambisense
  • Bipartite genome
  • Endogenous virus element
  • Evolution
  • High-throughput sequencing
  • Lentinula edodes
  • Mymonaviridae
  • Negative-strand RNA virus
  • Phenuiviridae
  • Shitake mushroom

ASJC Scopus subject areas

  • Virology

Cite this

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abstract = "There is still limited information on the diversity of (−)ssRNA viruses that infect fungi. Here, we have discovered two novel (−)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (−)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (−)ssRNA viruses in fungi.",
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AU - Hyoudou, Kiwamu

AU - Ida Bagus, Andika

AU - Suzuki, Nobuhiro

AU - Kondo, Hideki

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