Two kinds of active factor in crab hatch water

Ovigerous-Hair Stripping Substance (OHSS) and a proteinase

M. Saigusa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The embryos of intertidal and estuarine crabs are clustered on the ovigerous seta of the female, where they are ventilated for 2-4 weeks by the female's abdomen. When the embryonic development is complete, hatching occurs and zoea larvae are released into the water. This study indicates that the crab hatch water (i.e., the filtered medium into which zoeas were released) contains at least two kinds of active substance: OHSS (ovigerous-hair stripping substance) and a proteolytic enzyme. Both factors were separated by gel filtration. Powdered fragments of egg capsule were digested by proteinase, suggesting that this enzyme actually acts on the egg capsule. But this activity was at a very low level compared with casein digestion. The proteinase might be digesting the thin, sticky layer enclosing the embryo and would not act on the thick, tough layer constituting the main component of the egg capsule. Therefore, a proteolysis of such low activity could not be expected to cause the egg capsule to rupture.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalBiological Bulletin
Volume191
Issue number2
Publication statusPublished - Oct 1996

Fingerprint

hair
hairs
Capsules
Ovum
crab
crabs
embryo (animal)
Peptide Hydrolases
proteinases
egg
Water
setae (animal)
enzymes
proteolysis
abdomen
embryo
casein
embryogenesis
Embryonic Structures
hatching

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Aquatic Science

Cite this

Two kinds of active factor in crab hatch water : Ovigerous-Hair Stripping Substance (OHSS) and a proteinase. / Saigusa, M.

In: Biological Bulletin, Vol. 191, No. 2, 10.1996, p. 234-240.

Research output: Contribution to journalArticle

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