Abstract
We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling.
| Original language | English |
|---|---|
| Pages (from-to) | 436-442 |
| Number of pages | 7 |
| Journal | Plant Biology |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 2013 |
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Keywords
- Cytosolic alkalisation
- Cytosolic calcium oscillation
- Guard cell
- Mitogen-activated protein kinase
- Reactive oxygen species
- Stomatal closure
- Yeast elicitor
ASJC Scopus subject areas
- Plant Science
- Ecology, Evolution, Behavior and Systematics
Cite this
Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells. / Salam, M. A.; Jammes, F.; Hossain, M. A.; Ye, W.; Nakamura, Yoshimasa; Mori, Izumi; Kwak, J. M.; Murata, Yoshiyuki.
In: Plant Biology, Vol. 15, No. 3, 05.2013, p. 436-442.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells
AU - Salam, M. A.
AU - Jammes, F.
AU - Hossain, M. A.
AU - Ye, W.
AU - Nakamura, Yoshimasa
AU - Mori, Izumi
AU - Kwak, J. M.
AU - Murata, Yoshiyuki
PY - 2013/5
Y1 - 2013/5
N2 - We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling.
AB - We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling.
KW - Cytosolic alkalisation
KW - Cytosolic calcium oscillation
KW - Guard cell
KW - Mitogen-activated protein kinase
KW - Reactive oxygen species
KW - Stomatal closure
KW - Yeast elicitor
UR - http://www.scopus.com/inward/record.url?scp=84876078084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876078084&partnerID=8YFLogxK
U2 - 10.1111/j.1438-8677.2012.00671.x
DO - 10.1111/j.1438-8677.2012.00671.x
M3 - Article
C2 - 23043299
AN - SCOPUS:84876078084
VL - 15
SP - 436
EP - 442
JO - Acta Botanica Neerlandica
JF - Acta Botanica Neerlandica
SN - 1435-8603
IS - 3
ER -