Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells

M. A. Salam, F. Jammes, M. A. Hossain, W. Ye, Yoshimasa Nakamura, Izumi Mori, J. M. Kwak, Yoshiyuki Murata

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We report that two mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate abscisic acid (ABA)-induced stomatal closure in Arabidopsis thaliana. Yeast elicitor (YEL) induced stomatal closure accompanied by intracellular reactive oxygen species (ROS) accumulation and cytosolic free calcium concentration ([Ca2+]cyt) oscillation. In this study, we examined whether these two MAP kinases are involved in YEL-induced stomatal closure using MAPKK inhibitors, PD98059 and U0126, and MAPK mutants, mpk9, mpk12 and mpk9 mpk12. Both PD98059 and U0126 inhibited YEL-induced stomatal closure. YEL induced stomatal closure in the mpk9 and mpk12 mutants but not in the mpk9 mpk12 mutant, suggesting that a MAPK cascade involving MPK9 and MPK12 functions in guard cell YEL signalling. However, YEL induced extracellular ROS production, intracellular ROS accumulation and cytosolic alkalisation in the mpk9, mpk12 and mpk9 mpk12 mutants. YEL induced [Ca2+]cyt oscillations in both wild type and mpk9 mpk12 mutant. These results suggest that MPK9 and MPK12 function redundantly downstream of extracellular ROS production, intracellular ROS accumulation, cytosolic alkalisation and [Ca2+]cyt oscillation in YEL-induced stomatal closure in Arabidopsis guard cells and are shared with ABA signalling.

Original languageEnglish
Pages (from-to)436-442
Number of pages7
JournalPlant Biology
Volume15
Issue number3
DOIs
Publication statusPublished - May 2013

Fingerprint

guard cells
mitogen-activated protein kinase
yeast
Arabidopsis
yeasts
protein
reactive oxygen species
mutants
calcium
oscillation
alkalinization
abscisic acid
mitogen-activated protein kinase kinase
elicitors
inhibitor
Arabidopsis thaliana
mutant

Keywords

  • Cytosolic alkalisation
  • Cytosolic calcium oscillation
  • Guard cell
  • Mitogen-activated protein kinase
  • Reactive oxygen species
  • Stomatal closure
  • Yeast elicitor

ASJC Scopus subject areas

  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells. / Salam, M. A.; Jammes, F.; Hossain, M. A.; Ye, W.; Nakamura, Yoshimasa; Mori, Izumi; Kwak, J. M.; Murata, Yoshiyuki.

In: Plant Biology, Vol. 15, No. 3, 05.2013, p. 436-442.

Research output: Contribution to journalArticle

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