Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana

Md A R Khokon, M. A. Salam, F. Jammes, W. Ye, M. A. Hossain, M. Uraji, Yoshimasa Nakamura, Izumi Mori, J. M. Kwak, Yoshiyuki Murata

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Methyl jasmonate (MeJA) and abscisic acid (ABA) signalling cascades share several signalling components in guard cells. We previously showed that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signalling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signalling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants, but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in wild-type plants. MeJA elicited reactive oxygen species (ROS) production and cytosolic alkalisation in guard cells of the mpk9-1, mpk12-1 and mpk9-1 mpk12-1 mutants, as well in wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca2+ concentration ([Ca2+]cyt) in the mpk9-1 mpk12-1 double mutant as well as wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Together, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalisation and [Ca2+]cyt elevation in guard cell MeJA signalling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signalling in guard cells.

Original languageEnglish
Pages (from-to)946-952
Number of pages7
JournalPlant Biology
Volume17
Issue number5
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

methyl jasmonate
guard cells
mitogen-activated protein kinase
Arabidopsis thaliana
abscisic acid
protein
mutants
anion
alkalinization
calcium
anions
reactive oxygen species
mitogen-activated protein kinase kinase
inhibitor
mutant

Keywords

  • Cytosolic alkalisation
  • Guard cell, mitogen-activated protein kinase
  • Methyl jasmonate
  • Reactive oxygen species
  • S-type anion channels
  • [Ca] oscillations

ASJC Scopus subject areas

  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana. / Khokon, Md A R; Salam, M. A.; Jammes, F.; Ye, W.; Hossain, M. A.; Uraji, M.; Nakamura, Yoshimasa; Mori, Izumi; Kwak, J. M.; Murata, Yoshiyuki.

In: Plant Biology, Vol. 17, No. 5, 01.09.2015, p. 946-952.

Research output: Contribution to journalArticle

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abstract = "Methyl jasmonate (MeJA) and abscisic acid (ABA) signalling cascades share several signalling components in guard cells. We previously showed that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signalling in Arabidopsis thaliana. In this study, we examined whether these two MAP kinases function in MeJA signalling using genetic mutants for MPK9 and MPK12 combined with a pharmacological approach. MeJA induced stomatal closure in mpk9-1 and mpk12-1 single mutants as well as wild-type plants, but not in mpk9-1 mpk12-1 double mutants. Consistently, the MAPKK inhibitor PD98059 inhibited the MeJA-induced stomatal closure in wild-type plants. MeJA elicited reactive oxygen species (ROS) production and cytosolic alkalisation in guard cells of the mpk9-1, mpk12-1 and mpk9-1 mpk12-1 mutants, as well in wild-type plants. Furthermore, MeJA triggered elevation of cytosolic Ca2+ concentration ([Ca2+]cyt) in the mpk9-1 mpk12-1 double mutant as well as wild-type plants. Activation of S-type anion channels by MeJA was impaired in mpk9-1 mpk12-1. Together, these results indicate that MPK9 and MPK12 function upstream of S-type anion channel activation and downstream of ROS production, cytosolic alkalisation and [Ca2+]cyt elevation in guard cell MeJA signalling, suggesting that MPK9 and MPK12 are key regulators mediating both ABA and MeJA signalling in guard cells.",
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AU - Jammes, F.

AU - Ye, W.

AU - Hossain, M. A.

AU - Uraji, M.

AU - Nakamura, Yoshimasa

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