Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase

Naohiro Inohara, Atsuko Iwamoto, Yoshinori Moriyama, Shoji Shimomura, Masatomo Maeda, Masamitsu Futai

Research output: Contribution to journalArticle

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Abstract

Arabidopsis thaliana has two genes (atpC1, atpC2) coding for γ subunits of chloroplast ATP synthase. The atpC1 and atpC2 were cloned and sequenced. They had no introns within the reading frames and coded for proteins of 373 and 386 amino acid residues, respectively, including putative transit sequences (50 and 60 amino acid residues, respectively). In contrast, the spinach γ subunit gene had two introns within the reading frame. The mature sequences coded by the two genes of A. thaliana (atpC1, 323 residues; atpC2, 326 residues) were homologous with that of spinach (J. Miki, M. Maeda, Y. Mukohata, and M. Futai (1988) FEBS Lett. 232, 221-226): the homologies of γ subunits coded by atpC1 and atpC2 were 72%, those of the subunits coded by atpC1 and spinach cDNA were 84%, and those of the proteins coded by atpC2 and spinach cDNA were 71%. Like the spinach subunit, the γ subunits coded by the two genes had unique regulatory domains not found in mitochondrial or bacterial subunits. Poly(A)+ mRNAs corresponding to atpC1 (1.5 kilo-bases) and atpC2 (2.5 kilobases) were detected in illuminated plants, the amount of the former being at least 140 times that of the latter. The atpC1 mRNA was not found in dark-adapted plants. Nuclear protein(s) specifically bound to the upstream region of atpC1 was detected by gel shift assay and its binding was shown to be inhibited by the GT-1 element of the gene encoding the ribulose-1,5-bisphosphate carboxylase small subunit, which is expressed under illumination (P. J. Green, S. A. Kay, and N. H. Chau (1987) EMBO J. 6, 2543-2549). Consistent with these findings, an increased amount of the γ subunit was detected immunochemically in illuminated plants.

Original languageEnglish
Pages (from-to)7333-7338
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number12
Publication statusPublished - Apr 25 1991
Externally publishedYes

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Chloroplast Proton-Translocating ATPases
Spinacia oleracea
Arabidopsis
Genes
Reading Frames
Introns
Complementary DNA
Ribulose-Bisphosphate Carboxylase
Amino Acids
Messenger RNA
Gene encoding
Nuclear Proteins
Assays
Proteins
Lighting
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inohara, N., Iwamoto, A., Moriyama, Y., Shimomura, S., Maeda, M., & Futai, M. (1991). Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase. Journal of Biological Chemistry, 266(12), 7333-7338.

Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase. / Inohara, Naohiro; Iwamoto, Atsuko; Moriyama, Yoshinori; Shimomura, Shoji; Maeda, Masatomo; Futai, Masamitsu.

In: Journal of Biological Chemistry, Vol. 266, No. 12, 25.04.1991, p. 7333-7338.

Research output: Contribution to journalArticle

Inohara, N, Iwamoto, A, Moriyama, Y, Shimomura, S, Maeda, M & Futai, M 1991, 'Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase', Journal of Biological Chemistry, vol. 266, no. 12, pp. 7333-7338.
Inohara N, Iwamoto A, Moriyama Y, Shimomura S, Maeda M, Futai M. Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase. Journal of Biological Chemistry. 1991 Apr 25;266(12):7333-7338.
Inohara, Naohiro ; Iwamoto, Atsuko ; Moriyama, Yoshinori ; Shimomura, Shoji ; Maeda, Masatomo ; Futai, Masamitsu. / Two genes, atpC1 and atpC2, for the 7 subunit of Arabidopsis thaliana chloroplast ATP synthase. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 12. pp. 7333-7338.
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abstract = "Arabidopsis thaliana has two genes (atpC1, atpC2) coding for γ subunits of chloroplast ATP synthase. The atpC1 and atpC2 were cloned and sequenced. They had no introns within the reading frames and coded for proteins of 373 and 386 amino acid residues, respectively, including putative transit sequences (50 and 60 amino acid residues, respectively). In contrast, the spinach γ subunit gene had two introns within the reading frame. The mature sequences coded by the two genes of A. thaliana (atpC1, 323 residues; atpC2, 326 residues) were homologous with that of spinach (J. Miki, M. Maeda, Y. Mukohata, and M. Futai (1988) FEBS Lett. 232, 221-226): the homologies of γ subunits coded by atpC1 and atpC2 were 72{\%}, those of the subunits coded by atpC1 and spinach cDNA were 84{\%}, and those of the proteins coded by atpC2 and spinach cDNA were 71{\%}. Like the spinach subunit, the γ subunits coded by the two genes had unique regulatory domains not found in mitochondrial or bacterial subunits. Poly(A)+ mRNAs corresponding to atpC1 (1.5 kilo-bases) and atpC2 (2.5 kilobases) were detected in illuminated plants, the amount of the former being at least 140 times that of the latter. The atpC1 mRNA was not found in dark-adapted plants. Nuclear protein(s) specifically bound to the upstream region of atpC1 was detected by gel shift assay and its binding was shown to be inhibited by the GT-1 element of the gene encoding the ribulose-1,5-bisphosphate carboxylase small subunit, which is expressed under illumination (P. J. Green, S. A. Kay, and N. H. Chau (1987) EMBO J. 6, 2543-2549). Consistent with these findings, an increased amount of the γ subunit was detected immunochemically in illuminated plants.",
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