Two detoxification mechanisms by external malate detoxification and anti-peroxidation enzymes cooperatively confer aluminum tolerance in the roots of wheat (Triticum aestivum L.)

Anjali Aggarwal, Bunichi Ezaki, Bhumi Nath Tripathi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Present study demonstrates an antioxidant defense system against Al-induced oxidative damage and a role of external malate detoxification in providing Al-resistance in an Al resistant genotype of wheat, Raj 3077. Al dependent growth inhibition was higher in Raj 4120 (Al-sensitive) in comparison to Raj 3077. Oxidative damage indicators such as lipid peroxidation and H2O2 content were measured in the roots of both genotypes. However, these parameters were higher in the shoots of Raj 4120 indicating presence of oxidative stress in there. Antioxidant enzymes such as SOD (EC 1.15.1.9), CAT (EC 1.11.1.6) and APX (EC 1.11.1.11) played important roles in providing resistance to the Raj 3077 against Al-induced oxidative stress. Inefficient activation of the antioxidant enzymes in the roots of Raj 4120 suggested that a severe oxidative damage was occurred in root and a higher amount of translocated toxic Al ions to shoot consequently caused Al stress there. Interestingly, a higher CAT activity was found higher in the shoot of Raj 4120 than Raj 3077, but it seemed not to be enough to combat with Al-induced reactive oxygen species. Raj 3077 showed higher malate efflux and higher ALMT gene expression than Raj 4120. Further, root length of Raj 3077 seedlings was particularly affected in the presence of anion channel inhibitor, niflumic acid suggested that malate detoxification is required for providing Al-resistance in Raj 3077 in the root region. Thus, both Al induced antioxidant defensive and external malate detoxification systems provide strength to combat with Al toxicity in Raj 3077.

Original languageEnglish
Article number2965
Pages (from-to)43-54
Number of pages12
JournalEnvironmental and Experimental Botany
Volume120
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

peroxidation
malates
detoxification
aluminum
Triticum aestivum
tolerance
wheat
antioxidant
enzyme
enzymes
shoot
antioxidants
damage
shoots
genotype
oxidative stress
ascorbate peroxidase
anions
growth retardation
gene expression

Keywords

  • Al toxicity
  • ALMT1
  • Malate efflux
  • Oxidative stress
  • Raj 3077

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Agronomy and Crop Science

Cite this

Two detoxification mechanisms by external malate detoxification and anti-peroxidation enzymes cooperatively confer aluminum tolerance in the roots of wheat (Triticum aestivum L.). / Aggarwal, Anjali; Ezaki, Bunichi; Tripathi, Bhumi Nath.

In: Environmental and Experimental Botany, Vol. 120, 2965, 01.12.2015, p. 43-54.

Research output: Contribution to journalArticle

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