Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes

Akiwo Tani, Takeru Ishige, Yasuyoshi Sakai, Nobuo Kato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two genes encoding acyl-CoA dehydrogenases, acdA and acdB, arranged in tandem, were found in the chromosomal DNA of Acinetobacter sp. strain M-1. AcdA was purified from the parental strain and AcdB was purified from an Escherichia coli strain expressing the cloned gene. The substrate specificities of the two enzymes suggest that AcdA is a medium-chain acyl-CoA dehydrogenase and that AcdB is a long-chain acyl-CoA dehydrogenase. Characterization of n-alkane metabolism in Acinetobacter sp. strain M-1 has revealed parallel pathways as well as enzymes with overlapping specificities in a single pathway. The two acyl-CoA dehydrogenases described here provide another example of the physiological complexity underlying n-alkane utilization.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume94
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Acyl-CoA Dehydrogenases
Acinetobacter
Alkanes
Paraffins
Long-Chain Acyl-CoA Dehydrogenase
Acyl-CoA Dehydrogenase
Enzymes
Substrate Specificity
Genes
Gene encoding
Escherichia coli
Metabolism
DNA
Substrates
Oxidoreductases

Keywords

  • β-oxidation
  • Acinetobacter sp. strain M-1
  • acyl-CoA dehydrogenase
  • n-alkane

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes. / Tani, Akiwo; Ishige, Takeru; Sakai, Yasuyoshi; Kato, Nobuo.

In: Journal of Bioscience and Bioengineering, Vol. 94, No. 4, 2002, p. 326-329.

Research output: Contribution to journalArticle

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