Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes

Akio Tani; Takeru Ishige; Yasuyoshi Sakai; Nobuo Kato

doi:10.1263/jbb.94.326

Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes

Akio Tani, Takeru Ishige, Yasuyoshi Sakai, Nobuo Kato

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Two genes encoding acyl-CoA dehydrogenases, acdA and acdB, arranged in tandem, were found in the chromosomal DNA of Acinetobacter sp. strain M-1. AcdA was purified from the parental strain and AcdB was purified from an Escherichia coli strain expressing the cloned gene. The substrate specificities of the two enzymes suggest that AcdA is a medium-chain acyl-CoA dehydrogenase and that AcdB is a long-chain acyl-CoA dehydrogenase. Characterization of n-alkane metabolism in Acinetobacter sp. strain M-1 has revealed parallel pathways as well as enzymes with overlapping specificities in a single pathway. The two acyl-CoA dehydrogenases described here provide another example of the physiological complexity underlying n-alkane utilization.

Original language	English
Pages (from-to)	326-329
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	94
Issue number	4
DOIs	https://doi.org/10.1263/jbb.94.326
Publication status	Published - Jan 1 2002
Externally published	Yes

Keywords

Acinetobacter sp. strain M-1
acyl-CoA dehydrogenase
n-alkane
β-oxidation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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T1 - Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes

AU - Tani, Akio

AU - Ishige, Takeru

AU - Sakai, Yasuyoshi

AU - Kato, Nobuo

PY - 2002/1/1

Y1 - 2002/1/1

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AB - Two genes encoding acyl-CoA dehydrogenases, acdA and acdB, arranged in tandem, were found in the chromosomal DNA of Acinetobacter sp. strain M-1. AcdA was purified from the parental strain and AcdB was purified from an Escherichia coli strain expressing the cloned gene. The substrate specificities of the two enzymes suggest that AcdA is a medium-chain acyl-CoA dehydrogenase and that AcdB is a long-chain acyl-CoA dehydrogenase. Characterization of n-alkane metabolism in Acinetobacter sp. strain M-1 has revealed parallel pathways as well as enzymes with overlapping specificities in a single pathway. The two acyl-CoA dehydrogenases described here provide another example of the physiological complexity underlying n-alkane utilization.

KW - Acinetobacter sp. strain M-1

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KW - n-alkane

KW - β-oxidation

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Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes

Abstract

Keywords

ASJC Scopus subject areas

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