TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced Stomatal opening in Arabidopsis

Eigo Ando, Masato Ohnishi, Yin Wang, Tomonao Matsushita, Aiko Watanabe, Yuki Hayashi, Miho Fujii, Jian Feng Ma, Shin Ichiro Inoue, Toshinori Kinoshita

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Abstract

FLOWERING LOCUS T (FT) is the major regulatory component controlling photoperiodic floral transition. It is expressed in guard cells and affects blue light-induced stomatal opening induced by the blue-light receptor phototropins phot1 and phot2. Roles for other flowering regulators in stomatal opening have yet to be determined. We show in Arabidopsis (Arabidopsis thaliana) that TWIN SISTER OF FT (TSF), CONSTANS (CO), and GIGANTEA (GI) provide a positive effect on stomatal opening. TSF, which is the closest homolog of FT, was transcribed in guard cells, and light-induced stomatal opening was repressed in tsf-1, a T-DNA insertion mutant of TSF. Overexpression of TSF in a phot1 phot2 mutant background gave a constitutive open-stomata phenotype. Then, we examined whether CO and GI, which are upstream regulators of FT and TSF in photoperiodic flowering, are involved in stomatal opening. Similar to TSF, light-induced stomatal opening was suppressed in the GI and CO mutants gi-1 and co-1. A constitutive open-stomata phenotype was observed in GI and CO overexpressors with accompanying changes in the transcription of both FT and TSF. In photoperiodic flowering, photoperiod is sensed by photoreceptors such as the cryptochromes cry1 and cry2. We examined stomatal phenotypes in a cry1 cry2 mutant and in CRY2 overexpressors. Light-induced stomatal opening was suppressed in cry1 cry2, and the transcription of FT and TSF was down-regulated. In contrast, the stomata in CRY2 overexpressors opened even in the dark, and FT and TSF transcription was up-regulated. We conclude that the photoperiodic flowering components TSF, GI, and CO positively affect stomatal opening.

Original languageEnglish
Pages (from-to)1529-1538
Number of pages10
JournalPlant Physiology
Volume162
Issue number3
DOIs
Publication statusPublished - Jul 2013

Fingerprint

blue light
Arabidopsis
Light
Phenotype
Phototropins
Cryptochromes
stomata
flowering
mutants
transcription (genetics)
Photoperiod
guard cells
phenotype
photoreceptors
Arabidopsis thaliana
photoperiod
receptors
DNA

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Ando, E., Ohnishi, M., Wang, Y., Matsushita, T., Watanabe, A., Hayashi, Y., ... Kinoshita, T. (2013). TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced Stomatal opening in Arabidopsis. Plant Physiology, 162(3), 1529-1538. https://doi.org/10.1104/pp.113.217984

TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced Stomatal opening in Arabidopsis. / Ando, Eigo; Ohnishi, Masato; Wang, Yin; Matsushita, Tomonao; Watanabe, Aiko; Hayashi, Yuki; Fujii, Miho; Ma, Jian Feng; Inoue, Shin Ichiro; Kinoshita, Toshinori.

In: Plant Physiology, Vol. 162, No. 3, 07.2013, p. 1529-1538.

Research output: Contribution to journalArticle

Ando, E, Ohnishi, M, Wang, Y, Matsushita, T, Watanabe, A, Hayashi, Y, Fujii, M, Ma, JF, Inoue, SI & Kinoshita, T 2013, 'TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced Stomatal opening in Arabidopsis', Plant Physiology, vol. 162, no. 3, pp. 1529-1538. https://doi.org/10.1104/pp.113.217984
Ando, Eigo ; Ohnishi, Masato ; Wang, Yin ; Matsushita, Tomonao ; Watanabe, Aiko ; Hayashi, Yuki ; Fujii, Miho ; Ma, Jian Feng ; Inoue, Shin Ichiro ; Kinoshita, Toshinori. / TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced Stomatal opening in Arabidopsis. In: Plant Physiology. 2013 ; Vol. 162, No. 3. pp. 1529-1538.
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abstract = "FLOWERING LOCUS T (FT) is the major regulatory component controlling photoperiodic floral transition. It is expressed in guard cells and affects blue light-induced stomatal opening induced by the blue-light receptor phototropins phot1 and phot2. Roles for other flowering regulators in stomatal opening have yet to be determined. We show in Arabidopsis (Arabidopsis thaliana) that TWIN SISTER OF FT (TSF), CONSTANS (CO), and GIGANTEA (GI) provide a positive effect on stomatal opening. TSF, which is the closest homolog of FT, was transcribed in guard cells, and light-induced stomatal opening was repressed in tsf-1, a T-DNA insertion mutant of TSF. Overexpression of TSF in a phot1 phot2 mutant background gave a constitutive open-stomata phenotype. Then, we examined whether CO and GI, which are upstream regulators of FT and TSF in photoperiodic flowering, are involved in stomatal opening. Similar to TSF, light-induced stomatal opening was suppressed in the GI and CO mutants gi-1 and co-1. A constitutive open-stomata phenotype was observed in GI and CO overexpressors with accompanying changes in the transcription of both FT and TSF. In photoperiodic flowering, photoperiod is sensed by photoreceptors such as the cryptochromes cry1 and cry2. We examined stomatal phenotypes in a cry1 cry2 mutant and in CRY2 overexpressors. Light-induced stomatal opening was suppressed in cry1 cry2, and the transcription of FT and TSF was down-regulated. In contrast, the stomata in CRY2 overexpressors opened even in the dark, and FT and TSF transcription was up-regulated. We conclude that the photoperiodic flowering components TSF, GI, and CO positively affect stomatal opening.",
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AU - Ohnishi, Masato

AU - Wang, Yin

AU - Matsushita, Tomonao

AU - Watanabe, Aiko

AU - Hayashi, Yuki

AU - Fujii, Miho

AU - Ma, Jian Feng

AU - Inoue, Shin Ichiro

AU - Kinoshita, Toshinori

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AB - FLOWERING LOCUS T (FT) is the major regulatory component controlling photoperiodic floral transition. It is expressed in guard cells and affects blue light-induced stomatal opening induced by the blue-light receptor phototropins phot1 and phot2. Roles for other flowering regulators in stomatal opening have yet to be determined. We show in Arabidopsis (Arabidopsis thaliana) that TWIN SISTER OF FT (TSF), CONSTANS (CO), and GIGANTEA (GI) provide a positive effect on stomatal opening. TSF, which is the closest homolog of FT, was transcribed in guard cells, and light-induced stomatal opening was repressed in tsf-1, a T-DNA insertion mutant of TSF. Overexpression of TSF in a phot1 phot2 mutant background gave a constitutive open-stomata phenotype. Then, we examined whether CO and GI, which are upstream regulators of FT and TSF in photoperiodic flowering, are involved in stomatal opening. Similar to TSF, light-induced stomatal opening was suppressed in the GI and CO mutants gi-1 and co-1. A constitutive open-stomata phenotype was observed in GI and CO overexpressors with accompanying changes in the transcription of both FT and TSF. In photoperiodic flowering, photoperiod is sensed by photoreceptors such as the cryptochromes cry1 and cry2. We examined stomatal phenotypes in a cry1 cry2 mutant and in CRY2 overexpressors. Light-induced stomatal opening was suppressed in cry1 cry2, and the transcription of FT and TSF was down-regulated. In contrast, the stomata in CRY2 overexpressors opened even in the dark, and FT and TSF transcription was up-regulated. We conclude that the photoperiodic flowering components TSF, GI, and CO positively affect stomatal opening.

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