Tunicamycin-induced cell death in the trigeminal ganglion is suppressed by nerve growth factor in the mouse embryo

Hiroyuki Ichikawa, Bing Ran Zhao, Mitsuhiro Kano, Yoshinaka Shimizu, Toshihiko Suzuki, Ruji Terayama, Saburo Matsuo, Tomosada Sugimoto

Research output: Contribution to journalArticle

Abstract

The effect of nerve growth factor (NGF) on tunicamycin (Tm)-treated neurons in the trigeminal ganglion was investigated by use of caspase-3 immunohistochemistry. In intact embryos at embryonic day 16.5, only a few caspase-3-immunoreactivity were detected in the ganglion neurons. Mean ± SE of the density of the immunoreactivity was 0.22 ± 0.03%. In contrast, the number of the immunoreactive neurons was increased at 24 h after injection of 0.5 μg Tm in 1 μl of 0.05 N NaOH solution into mouse embryos at embryonic day 15.5. The density of immunoreactivity was also increased (mean ± SE = 1.44 ± 0.11%) compared to intact and 0.05 N NaOH-treated embryos (mean ± SE = 0.35 ± 0.03%). The Tm treatment caused increase of the number of trigeminal neurons representing apoptotic profiles (intact, mean ± SE = 79.3 ± 8.5; 0.05 N NaOH, mean ± SE = 132 ± 11.5; 0.5 μg Tm, mean ± SE = 370.2 ± 64.8). In addition, NGF significantly prevented the increase of density of the immunoreactivity (mean ± SE = 0.54 ± 0.16%) and the number of apoptotic cells (mean ± SE = 146.2 ± 11.3). Saline application (without NGF) had no effect on Tm-induced increase of the immunoreactivity (mean ± SE = 1.78 ± 0.23%) or the apoptotic profiles (mean ± SE = 431.9 ± 80.5). These results indicate that Tm-induced cell death in the trigeminal ganglion is suppressed by NGF in the mouse embryo.

Original languageEnglish
Pages (from-to)461-467
Number of pages7
JournalCellular and molecular neurobiology
Volume30
Issue number3
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Caspase-3
  • Mouse embryo
  • Nerve growth factor
  • Sensory ganglion
  • Trigeminal ganglion
  • Tunicamycin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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  • Cite this

    Ichikawa, H., Zhao, B. R., Kano, M., Shimizu, Y., Suzuki, T., Terayama, R., Matsuo, S., & Sugimoto, T. (2010). Tunicamycin-induced cell death in the trigeminal ganglion is suppressed by nerve growth factor in the mouse embryo. Cellular and molecular neurobiology, 30(3), 461-467. https://doi.org/10.1007/s10571-009-9471-6