Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems

Eisaku Kondo, Ken Saito, Yuichi Tashiro, Kaeko Kamide, Shusei Uno, Tomoko Furuya, Masao Mashita, Kiichiro Nakajima, Tomoyuki Tsumuraya, Naoya Kobayashi, Masahiro Nishibori, Mitsune Tanimoto, Masayuki Matsushita

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Cell-penetrating peptides have gained attention owing to their promise in noninvasive delivery systems. Among the identified cell-penetrating peptides, the TAT peptide has been preferentially used for transduction into cells of diverse origins. However, this activity is nonselective between neoplastic and non-neoplastic cells. Here we describe artificial cell-penetrating peptides that are selectively and efficiently incorporated into human tumour cells, according to their lineage. Ten representative tumour lineage-homing cell-penetrating peptides were obtained by screening of a random peptide library constructed using messenger RNA display technology, and some of the isolates were further modified by amino-acid substitution. Their advantageous tumour cell-targeting ability is corroborated in an in vivo mouse model for imaging and growth suppression of metastatic xenoplant tumours. These cell-penetrating peptides are potentially useful for the efficient targeting of human neoplasms in a tumour origin-dependent manner, and provide a framework for the development of peptide-based anti-tumour technologies.

Original languageEnglish
Article number951
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012

Fingerprint

homing
Cell-Penetrating Peptides
peptides
Tumors
delivery
tumors
cells
Neoplasms
Cells
Peptide Library
Peptides
Artificial Cells
Technology
ribonucleic acids
neoplasms
Amino Acid Substitution
Screening
Substitution reactions
Display devices
mice

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Kondo, E., Saito, K., Tashiro, Y., Kamide, K., Uno, S., Furuya, T., ... Matsushita, M. (2012). Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. Nature Communications, 3, [951]. https://doi.org/10.1038/ncomms1952

Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. / Kondo, Eisaku; Saito, Ken; Tashiro, Yuichi; Kamide, Kaeko; Uno, Shusei; Furuya, Tomoko; Mashita, Masao; Nakajima, Kiichiro; Tsumuraya, Tomoyuki; Kobayashi, Naoya; Nishibori, Masahiro; Tanimoto, Mitsune; Matsushita, Masayuki.

In: Nature Communications, Vol. 3, 951, 2012.

Research output: Contribution to journalArticle

Kondo, E, Saito, K, Tashiro, Y, Kamide, K, Uno, S, Furuya, T, Mashita, M, Nakajima, K, Tsumuraya, T, Kobayashi, N, Nishibori, M, Tanimoto, M & Matsushita, M 2012, 'Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems', Nature Communications, vol. 3, 951. https://doi.org/10.1038/ncomms1952
Kondo, Eisaku ; Saito, Ken ; Tashiro, Yuichi ; Kamide, Kaeko ; Uno, Shusei ; Furuya, Tomoko ; Mashita, Masao ; Nakajima, Kiichiro ; Tsumuraya, Tomoyuki ; Kobayashi, Naoya ; Nishibori, Masahiro ; Tanimoto, Mitsune ; Matsushita, Masayuki. / Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. In: Nature Communications. 2012 ; Vol. 3.
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