Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction

Robert M. Hoffman, Shuuya Yano

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cancer cells require elevated amounts of methionine (MET) and arrest their growth under conditions of MET restriction (MR). This phenomenon is termed MET dependence. Fluorescence-activated cell sorting (FACS) first indicated that the MET-dependent SV40-transformed cancer cells were arrested in the S and G 2 phases of the cell cycle when under MR. This is in contrast to a G 1 -phase accumulation of cells, which occurs only in MET-supplemented medium at very high cell densities and which is similar to the G 1 cell-cycle block which occurs in cultures of normal fibroblasts at high density. When the human PC-3 prostate carcinoma cell line was cultured in MET-free, homocysteine-containing (MET HCY + ) medium, there was an extreme increment in DNA content without cell division indicating that the cells were blocked in S phase. Recombinant methioninase (rMETase) treatment of cancer cells also selectively trapped cancer cells in S/G 2 : The cell cycle phase of the cancer cells was visualized with the fluorescence ubiquitination cell cycle indicator (FUCCI). At the time of rMETase-induced S/G 2 -phase trap, identified by the cancer cells’ green fluorescence by FUCCI imaging, the cancer cells were administered S-phase-dependent chemotherapy drugs, which interact with DNA or block DNA synthesis such as doxorubicin, cisplatin, or 5-fluorouracil (5-FU) and which were highly effective in killing the cancer cells. In contrast, treatment of cancer cells with drugs in the presence of MET, only led to the majority of the cancer cell population being blocked in G 0 /G 1 phase, identified by the cancer cells becoming red fluorescent in the FUCCI system. The G 0 /G 1 blocked cells were resistant to the chemotherapy. MR has the potential for highly effective cell-cycle-based treatment strategy for cancer in the clinic.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages49-60
Number of pages12
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1866
ISSN (Print)1064-3745

Fingerprint

Cell Cycle Checkpoints
Methionine
Neoplasms
Cell Cycle
Ubiquitination
Fluorescence
S Phase
DNA
Drug Therapy
Gastrin-Secreting Cells
Homocysteine
Fluorouracil
Pharmaceutical Preparations
Cell Division
Doxorubicin
Cisplatin
Prostate
Flow Cytometry
Therapeutics
Fibroblasts

Keywords

  • Arrest
  • Cancer cells
  • Cell cycle
  • Methionine dependence
  • Methionine restriction
  • S/G phase, chemotherapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hoffman, R. M., & Yano, S. (2019). Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction In Methods in Molecular Biology (pp. 49-60). (Methods in Molecular Biology; Vol. 1866). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8796-2_5

Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction . / Hoffman, Robert M.; Yano, Shuuya.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 49-60 (Methods in Molecular Biology; Vol. 1866).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hoffman, RM & Yano, S 2019, Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1866, Humana Press Inc., pp. 49-60. https://doi.org/10.1007/978-1-4939-8796-2_5
Hoffman RM, Yano S. Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction In Methods in Molecular Biology. Humana Press Inc. 2019. p. 49-60. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8796-2_5
Hoffman, Robert M. ; Yano, Shuuya. / Tumor-specific S/G 2 -Phase cell cycle arrest of cancer cells by methionine restriction Methods in Molecular Biology. Humana Press Inc., 2019. pp. 49-60 (Methods in Molecular Biology).
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