Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma

Beyhan Cengiz, Esra Gunduz, Mehmet Gunduz, Levent Bekir Beder, Ryo Tamamura, Cahit Bagci, Noboru Yamanaka, Kenji Shimizu, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Our previous study showed high frequency of allelic loss at chromosome 2q37 region in oral cancer. This location contains several candidate tumor suppressor genes such as PPP1R7, ILKAP, DTYMK and ING5. We previously showed 3 members of inhibitor of growth (ING) family, ING1, ING3 and ING4 as tumor suppressor gene in head and neck cancer. As ING5 shows high homology with other members of ING genes including highly conserved carboxy-terminal plant homeodomain and nuclear localization signal, we first picked up ING5 and examined it as a possible tumor suppressor in oral cancer. For this aim, mutation and mRNA expression status of ING5 in paired normal and oral squamous cell carcinoma samples were examined by reverse transcription polymerase chain reaction (RT-PCR) and sequencing. Three missense mutations located within leucine zipper like (LZL) finger and novel conserved region (NCR) domains in ING5 protein were detected, probably abrogating its normal function. We also found 5 different alternative splicing variants of ING5. Then, we examined mRNA level of ING5 by quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) analysis, which demonstrated decreased expression of ING5 mRNA in 61% of the primary tumors as compared to the matched normal samples. In conclusion, tumor-specific mutation and downregulation of ING5 mRNA suggested it as a tumor suppressor gene in oral squamous cell carcinoma.

Original languageEnglish
Pages (from-to)2088-2094
Number of pages7
JournalInternational Journal of Cancer
Volume127
Issue number9
DOIs
Publication statusPublished - Nov 1 2010

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Squamous Cell Carcinoma
Down-Regulation
Tumor Suppressor Genes
Growth Inhibitors
Messenger RNA
Mutation
Mouth Neoplasms
Reverse Transcription
Neoplasms
Leucine Zippers
Nuclear Localization Signals
Polymerase Chain Reaction
Loss of Heterozygosity
Alternative Splicing
Missense Mutation
Head and Neck Neoplasms
Fingers
Chromosomes
Genes
Proteins

Keywords

  • ING1
  • ING5
  • mutation
  • oral cancer
  • splicing variant
  • tumor suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma. / Cengiz, Beyhan; Gunduz, Esra; Gunduz, Mehmet; Beder, Levent Bekir; Tamamura, Ryo; Bagci, Cahit; Yamanaka, Noboru; Shimizu, Kenji; Nagatsuka, Hitoshi.

In: International Journal of Cancer, Vol. 127, No. 9, 01.11.2010, p. 2088-2094.

Research output: Contribution to journalArticle

Cengiz, B, Gunduz, E, Gunduz, M, Beder, LB, Tamamura, R, Bagci, C, Yamanaka, N, Shimizu, K & Nagatsuka, H 2010, 'Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma', International Journal of Cancer, vol. 127, no. 9, pp. 2088-2094. https://doi.org/10.1002/ijc.25224
Cengiz, Beyhan ; Gunduz, Esra ; Gunduz, Mehmet ; Beder, Levent Bekir ; Tamamura, Ryo ; Bagci, Cahit ; Yamanaka, Noboru ; Shimizu, Kenji ; Nagatsuka, Hitoshi. / Tumor-specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma. In: International Journal of Cancer. 2010 ; Vol. 127, No. 9. pp. 2088-2094.
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