Tumor-related protein, the squamous cell carcinoma antigen binds to the intracellular protein carbonyl reductase

Akihiro Murakami, Chikako Fukushima, Keiko Yositomi, Kotaro Sueoka, Shugo Nawata, Masanori Fujimoto, Kazuyuki Nakamura, Norihiro Sugino

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Squamous cell carcinoma antigen (SCCA) is a useful tumor marker for diagnosis and management of squamous cell carcinoma. Recent reports have shown that SCCA can influence the invasion or metastasis of cancer cells. However, it remained unclear how SCCA acts to mediate these biological functions. To solve this question, at first, SCCA1-and SCCA2-glutathione S-transferase fusion protein were used to purify a protein which binds to SCCA1 or SCCA2, and the combined protein was identified by proteomic analysis. Secondly, immunocytochemical and immunohistochemical analyses were performed to investigate the localization of this protein. Third, Western blotting was performed to analyze the expression levels of this protein in keratinocytes and six kinds of uterine squamous cell carcinoma cell lines. Both SCCA1 and SCCA2 molecules bind to the cytoplasmic protein, which was identified to be carbonyl reductase (CR). The immunostaining analyses revealed that CR is located in the cytoplasm of keratinocytes and the normal squamous epithelial cells of the uterine cervix as well as SCCA1 and SCCA2. The CR expression levels in six kinds of squamous cell carcinoma cell lines were lower compared to those in keratinocytes. In conclusion, CR binds to SCCA1 and SCCA2 and they are co-located in the same layer of the squamous epithelium, suggesting that CR may collaborate with SCCA1 and SCCA2 to mediate cancer behavior such as invasion or metastasis.

Original languageEnglish
Pages (from-to)1395-1400
Number of pages6
JournalInternational journal of oncology
Volume36
Issue number6
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

Keywords

  • Carbonyl reductase
  • Cervical cancer
  • SCC antigen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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