Tumor necrosis factor-alpha gene (TNF-α) -1031/ -863, -857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese

Yoshihiko Soga, Fusanori Nishimura, Hideki Ohyama, Hiroshi Maeda, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Objectives: Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-α, -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-α production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-α or IL-1β SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-α gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1β gene (-511, -31, +3953). Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-α and IL-1β SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-α and IL-1β production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects. Results: TNF-α production in TNF-α -1031/ -863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects. Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-α -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

Original languageEnglish
Pages (from-to)524-531
Number of pages8
JournalJournal of Clinical Periodontology
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Chronic Periodontitis
Single Nucleotide Polymorphism
Tumor Necrosis Factor-alpha
Periodontitis
Genes
Interleukin-1
Healthy Volunteers
Alleles
Cytokines
Restriction Fragment Length Polymorphisms
Population
Monocytes

Keywords

  • Adult periodontitis
  • Interleukin-1 beta (IL-1β)
  • Single-nucleotide polymorphisms (SNPs)
  • Tumor necrosis factor-alpha (TNF-α)

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tumor necrosis factor-alpha gene (TNF-α) -1031/ -863, -857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese. / Soga, Yoshihiko; Nishimura, Fusanori; Ohyama, Hideki; Maeda, Hiroshi; Takashiba, Shogo; Murayama, Yoji.

In: Journal of Clinical Periodontology, Vol. 30, No. 6, 06.2003, p. 524-531.

Research output: Contribution to journalArticle

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abstract = "Objectives: Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-α, -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-α production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-α or IL-1β SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-α gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1β gene (-511, -31, +3953). Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-α and IL-1β SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-α and IL-1β production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects. Results: TNF-α production in TNF-α -1031/ -863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects. Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-α -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.",
keywords = "Adult periodontitis, Interleukin-1 beta (IL-1β), Single-nucleotide polymorphisms (SNPs), Tumor necrosis factor-alpha (TNF-α)",
author = "Yoshihiko Soga and Fusanori Nishimura and Hideki Ohyama and Hiroshi Maeda and Shogo Takashiba and Yoji Murayama",
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T1 - Tumor necrosis factor-alpha gene (TNF-α) -1031/ -863, -857 single-nucleotide polymorphisms (SNPs) are associated with severe adult periodontitis in Japanese

AU - Soga, Yoshihiko

AU - Nishimura, Fusanori

AU - Ohyama, Hideki

AU - Maeda, Hiroshi

AU - Takashiba, Shogo

AU - Murayama, Yoji

PY - 2003/6

Y1 - 2003/6

N2 - Objectives: Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-α, -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-α production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-α or IL-1β SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-α gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1β gene (-511, -31, +3953). Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-α and IL-1β SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-α and IL-1β production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects. Results: TNF-α production in TNF-α -1031/ -863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects. Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-α -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

AB - Objectives: Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) participate in the establishment of inflammatory lesions in periodontitis. High production of these cytokines may relate to the severity of periodontitis. There have already been several studies examining the association between periodontitis and single nucleotide polymorphisms (SNPs) that affect cytokine productivity. Recently, new SNPs of TNF-α, -1031, -863 and -857, variants of which are observed in a relatively large proportion in Japanese, have been identified. The variant alleles of these SNPs have been suggested to be related to high TNF-α production. For a better understanding of the genetic factors associated with the severity of periodontitis, further analysis including these newly identified SNPs is essential. In addition, previous reports on TNF-α or IL-1β SNPs associated with periodontitis were mainly for Caucasian populations. Therefore, the aim of this study is to examine the association between severe periodontitis in Japanese and the following SNPs: five in the TNF-α gene promoter (-1031, -863, -857, -308, -238) and three in the IL-1β gene (-511, -31, +3953). Material and Methods: A total of 128 Japanese individuals were enrolled in this study. They were 64 patients with severe adult periodontitis and 64 healthy subjects. TNF-α and IL-1β SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism for all subjects. TNF-α and IL-1β production from LPS-stimulated monocytes/macrophages was also measured for 15 healthy male subjects. Results: TNF-α production in TNF-α -1031/ -863 (linkage disequilibrated) or -857 SNP variant allele carriers tended to be elevated, and the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs among severe periodontitis patients was significantly higher than in healthy subjects. Conclusion: Since the frequency of subjects who carried at least one variant allele in TNF-α -1031, -863 or -857 SNPs was higher in periodontitis patients than in healthy subjects, TNF-α -1031, -863 and -857 SNPs appear to be associated with severe adult periodontitis in Japanese populations.

KW - Adult periodontitis

KW - Interleukin-1 beta (IL-1β)

KW - Single-nucleotide polymorphisms (SNPs)

KW - Tumor necrosis factor-alpha (TNF-α)

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