Tumor Necrosis Factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus

M. J. Siemieniuch, K. Ogrodowska, H. Ohgawara, D. J. Skarzynski, K. Okuda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Tumor Necrosis Factor-alpha (TNFa) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNFα role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNFa in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNFα and its receptors (TNFR1 and TNFR2), and TNFa protein content at different stages of the estrous cycle were investigated. Additionally, TNFα involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNFa and TNFR1 were the highest at diestrus (P < 0.05). TNFα protein expression was the lowest at interestrus (P < 0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNFα at a dose of 1 ng/ml significantly increased PGF2a secretion at estrus (P < 0.01) and PGE2 secretion at diestrus (P < 0.001) after 12h incubation. Overall findings indicate that TNFα locally produced in the cat's uterus, stimulates PG secretion in an estrous cycle-related manner.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalPolish Journal of Veterinary Sciences
Volume13
Issue number4
DOIs
Publication statusPublished - Dec 1 2010

Keywords

  • Cat
  • Prostaglandin
  • Tumor necrosis factor-alpha
  • Uterus

ASJC Scopus subject areas

  • veterinary(all)

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