Abstract
The aim of this study was to investigate whether functional tumor necrosis factor-α (TNFα) receptors are present in the granulosa cells and the cells of theca interna (theca cells), obtained from bovine follicles classified into one of three groups. Each group was defined as either small vesicular ovarian follicles (small follicles; 3-5 mm in diameter), preovulatory mature ovarian follicles (preovulatory follicles) or atretic follicles (12-18 mm) according to gross examination of the corpus luteum in the epsilateral or contralateral ovary and the uterus (size, color, consistency and mucus), and the ratio of progesterone (P4) and estradiol-17β (E2) concentrations in follicular fluid. A Scatchard analysis showed the presence of a high-affinity binding site on both granulosa and theca cells from all follicles examined (dissociation constant: 4.7 ± 0.15 to 6.9 ± 1.40 nM). Moreover, TNFα receptor concentrations in granulosa and theca cells obtained from atretic follicles were significantly higher than those in the cells from preovulatory follicles (P2 secretion in a dose-dependent fashion (P4 secretion. In addition, rhTNFα inhibited follicle stimulating hormone-, forskolin- or dibutylyl cyclic AMP-induced P4 and E2 secretion by the cells (P
| Original language | English |
|---|---|
| Pages (from-to) | 441-449 |
| Number of pages | 9 |
| Journal | Journal of Reproduction and Development |
| Volume | 49 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2003 |
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Keywords
- Bovine
- Ovary
- Receptor
- Tumor necrosis factor-α
ASJC Scopus subject areas
- Animal Science and Zoology
- Reproductive Medicine
Cite this
Tumor necrosis factor-α (TNFα) inhibits progesterone and estradiol-17β production from cultured granulosa cells : Presence of TNFα receptors in bovine granulosa and theca cells. / Sakumoto, Ryosuke; Shibaya, Masami; Okuda, Kiyoshi.
In: Journal of Reproduction and Development, Vol. 49, No. 6, 2003, p. 441-449.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor necrosis factor-α (TNFα) inhibits progesterone and estradiol-17β production from cultured granulosa cells
T2 - Presence of TNFα receptors in bovine granulosa and theca cells
AU - Sakumoto, Ryosuke
AU - Shibaya, Masami
AU - Okuda, Kiyoshi
PY - 2003
Y1 - 2003
N2 - The aim of this study was to investigate whether functional tumor necrosis factor-α (TNFα) receptors are present in the granulosa cells and the cells of theca interna (theca cells), obtained from bovine follicles classified into one of three groups. Each group was defined as either small vesicular ovarian follicles (small follicles; 3-5 mm in diameter), preovulatory mature ovarian follicles (preovulatory follicles) or atretic follicles (12-18 mm) according to gross examination of the corpus luteum in the epsilateral or contralateral ovary and the uterus (size, color, consistency and mucus), and the ratio of progesterone (P4) and estradiol-17β (E2) concentrations in follicular fluid. A Scatchard analysis showed the presence of a high-affinity binding site on both granulosa and theca cells from all follicles examined (dissociation constant: 4.7 ± 0.15 to 6.9 ± 1.40 nM). Moreover, TNFα receptor concentrations in granulosa and theca cells obtained from atretic follicles were significantly higher than those in the cells from preovulatory follicles (P2 secretion in a dose-dependent fashion (P4 secretion. In addition, rhTNFα inhibited follicle stimulating hormone-, forskolin- or dibutylyl cyclic AMP-induced P4 and E2 secretion by the cells (P
AB - The aim of this study was to investigate whether functional tumor necrosis factor-α (TNFα) receptors are present in the granulosa cells and the cells of theca interna (theca cells), obtained from bovine follicles classified into one of three groups. Each group was defined as either small vesicular ovarian follicles (small follicles; 3-5 mm in diameter), preovulatory mature ovarian follicles (preovulatory follicles) or atretic follicles (12-18 mm) according to gross examination of the corpus luteum in the epsilateral or contralateral ovary and the uterus (size, color, consistency and mucus), and the ratio of progesterone (P4) and estradiol-17β (E2) concentrations in follicular fluid. A Scatchard analysis showed the presence of a high-affinity binding site on both granulosa and theca cells from all follicles examined (dissociation constant: 4.7 ± 0.15 to 6.9 ± 1.40 nM). Moreover, TNFα receptor concentrations in granulosa and theca cells obtained from atretic follicles were significantly higher than those in the cells from preovulatory follicles (P2 secretion in a dose-dependent fashion (P4 secretion. In addition, rhTNFα inhibited follicle stimulating hormone-, forskolin- or dibutylyl cyclic AMP-induced P4 and E2 secretion by the cells (P
KW - Bovine
KW - Ovary
KW - Receptor
KW - Tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=0347093365&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347093365&partnerID=8YFLogxK
U2 - 10.1262/jrd.49.441
DO - 10.1262/jrd.49.441
M3 - Article
C2 - 14967894
AN - SCOPUS:0347093365
VL - 49
SP - 441
EP - 449
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 6
ER -