Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor-κB activation in MC3T3-E1 osteoblastic cells

Yaqiong Yu, Di Yang, Lihong Qiu, Hirohiko Okamura, Jiajie Guo, Tatsuji Haneji

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Osteoblasts produce various types of cytokines under pathological conditions and control osteoclast differentiation. Tumor necrosis factor-α (TNF-α) has been demonstrated to exert complex effects in osteoblasts under local inflammatory conditions, including in periodontal and periapical diseases. Interleukin-34 (IL-34) has been recently identified as a novel regulatory factor for the differentiation and function of osteoclasts. The present study provides the first evidence, to the best of our knowledge, that the expression of IL-34 is induced by TNF-α through nuclear factor-κB (NF-κB) activation in MC3T3-E1 osteoblastic cells. TNF-α induced IL-34 expression in a dose- and time-dependent manner. Immunocytochemistry with an NF-κB antibody demonstrated that NF-κB was mainly localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation of NF-κB from the cytoplasm to the nucleus was observed in the cells treated with TNF-α for 15 min. Translocation and transcriptional activity of NF-κB were also determined by western blotting and a luciferase reporter assay, respectively. Pretreatment with 100 μM CAPE, an inhibitor of NF-κB, significantly inhibited TNF-α-induced IL-34 expression. These results indicate that TNF-α induces IL-34 expression via NF-κB in osteoblasts.

Original languageEnglish
Pages (from-to)1371-1376
Number of pages6
JournalMolecular Medicine Reports
Volume10
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Interleukins
Tumor Necrosis Factor-alpha
Chemical activation
Osteoblasts
Periapical Diseases
Cytoplasm
RANK Ligand
Periodontal Diseases
Osteoclasts
Luciferases
Assays
Western Blotting
Immunohistochemistry
Cytokines
Antibodies

Keywords

  • Interleukin-34
  • Nuclear factor-κB
  • Osteoblast
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Cancer Research
  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Oncology
  • Medicine(all)

Cite this

Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor-κB activation in MC3T3-E1 osteoblastic cells. / Yu, Yaqiong; Yang, Di; Qiu, Lihong; Okamura, Hirohiko; Guo, Jiajie; Haneji, Tatsuji.

In: Molecular Medicine Reports, Vol. 10, No. 3, 2014, p. 1371-1376.

Research output: Contribution to journalArticle

Yu, Yaqiong ; Yang, Di ; Qiu, Lihong ; Okamura, Hirohiko ; Guo, Jiajie ; Haneji, Tatsuji. / Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor-κB activation in MC3T3-E1 osteoblastic cells. In: Molecular Medicine Reports. 2014 ; Vol. 10, No. 3. pp. 1371-1376.
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