Tumor necrosis factor-α can induce Langhans-type multinucleated giant cell formation derived from myeloid dendritic cells

Kozo Yasui, Masato Yashiro, Mitsuru Tsuge, Yoichi Kondo, Yukie Saito, Yoshiharu Nagaoka, Nobuko Yamashita, Tsuneo Morishima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The formation of the rich cellular features of MGCs, where the nuclei are arranged circularly at the periphery of the cell (morphologically epithelioid; Langhans-type), is assumed to be associated with any granulomatous disease. The mechanism by which TNF controls the formation of human MGCs in vitro was investigated, focusing on the effect of the TNF-neutralizing antibody. Peripheral blood monocytes were isolated with mAb-coated immunologic magnetic beads and cultured for 10 days in the presence of 20 ng/mL GM-CSF and 10 ng/mL IL-4. These cells were further incubated in the presence of TNF-α with/without its blockade antibodies for 14 days. Myeloid DCs can be generated from peripheral blood monocytes, and both IL-4 and GM-CSF can provide sufficient stimulus for their differentiation. The formation of MGC can be induced in the presence of TNF-α. This reaction was prohibited by the presence of the TNF-neutralizing antibody but not by the presence of anti-TNF receptor II antibody. The activation of Rho and focal adhesion kinases induced by TNF-α stimulation might be linked to cell assembling and the formation of Langhans-type MGCs. MGCs can produce only small amounts of superoxide anions compared to isolated macrophages such as myeloid DCs.

Original languageEnglish
Pages (from-to)809-816
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume55
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Dendritic cell
  • Granulocyte/macrophage colony-stimulating factor
  • Interleukin-4
  • Monocyte

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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