Tumor necrosis factor-α and its receptor in the corpus luteum of pregnant cows

Ryosuke Sakumoto, Shuko Murakami, Hisashi Kishi, Kosuke Iga, Akira Okano, Kiyoshi Okuda

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The objective of this study was to investigate the presence of tumor necrosis factor (TNF)-α mRNA and TNF-α receptors in the bovine corpus luteum (CL) during the gestation period. TNF-α mRNA and TNF-α receptors were determined on bovine CL from pregnant cows at three stages: trimester I (fetal crown-rum p length; 6-20 cm), trimester II (25-45 cm) and trimester (50-80 cm). TNF-α mRNA was detected by an RT-PCR analysis in the CL of all stages of gestation. A Scatchard analysis revealed the presence of a high- affinity binding site (Kd; 5.1-6.9 nM) in the CL membranes collected at each stage of gestation. Furthermore, the concentrations of TNF-α receptors in the CL of trimesters I (24.0 ± 1.95 pmol/mg protein) and III (21.6 ± 2.39 pmol/mg protein) of gestation were significantly higher than the concentration in trimester II (14.9 ± 2.07 pmol/mg protein) (P < 0.05). These results indicate that TNF-α is locally produced and that TNF-α receptors are present in bovine CL during the gestation period, and suggest that TNF-α plays one or more roles as a paracrine factor in regulating bovine CL function during the entire gestation period. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalMolecular Reproduction and Development
Issue number4
Publication statusPublished - 2000


  • Corpus luteum
  • Pregnancy
  • Receptor
  • TNF-α

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology


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