Tumor growth limited to subcutaneous site vs tumor growth in pulmonary site exhibit differential effects on systemic immunities

Junko Masuda, Eiji Takayama, Warren Strober, Ayano Satoh, Yuji Morimoto, Yasuko Honjo, Tatsuo Ichinohe, Shin Ichi Tokuno, Toshiaki Ishizuka, Takahiro Nakata, Akifumi Mizutani, Naoki Umemura, Atsushi Kitani, Ivan J. Fuss, Tsukasa Shigehiro, Harumi Kawaki, Masako Mizuno-Kamiya, Nobuo Kondoh, Masaharu Seno

Research output: Contribution to journalArticle

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Abstract

To evaluate systemic immunity associated with tumor growth limited to a subcutaneous site versus growth proceeding at multiple tumor sites, we established syngeneic mouse subcutaneous and pulmonary tumor models by local subcutaneous and intravenous injection of colon carcinoma CT26 cells. We found that splenic myeloid-derived suppressor cell (MDSC) levels were significantly increased in the subcutaneous tumor model but not in the pulmonary tumor model. Furthermore, both CD4+ and CD8+ T cells as well as CD4+ Foxp3+ T cells were significantly decreased in the subcutaneous tumor model and were largely unchanged in the pulmonary tumor model. In addition, the subcutaneous model, but not the pulmonary model, displayed a Th1 polarization bias. This bias was characterized by decreased IL-4, IL-9, and IL-10 production, whereas the pulmonary model displayed increased production of IL-10. These results suggest that the mode of tumor development has differential effects on systemic immunity that may, in turn, influence approaches to treatment of cancer patients.

Original languageEnglish
Pages (from-to)449-455
Number of pages7
JournalOncology Reports
Volume38
Issue number1
DOIs
Publication statusPublished - 2017

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Immunity
Lung
Growth
Neoplasms
Interleukin-10
Interleukin-9
T-Lymphocytes
Subcutaneous Injections
Intravenous Injections
Interleukin-4
Colon
Carcinoma

Keywords

  • Colon carcinoma
  • Cytokine
  • Myeloid-derived suppressor cell
  • Regulatory T cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Tumor growth limited to subcutaneous site vs tumor growth in pulmonary site exhibit differential effects on systemic immunities. / Masuda, Junko; Takayama, Eiji; Strober, Warren; Satoh, Ayano; Morimoto, Yuji; Honjo, Yasuko; Ichinohe, Tatsuo; Tokuno, Shin Ichi; Ishizuka, Toshiaki; Nakata, Takahiro; Mizutani, Akifumi; Umemura, Naoki; Kitani, Atsushi; Fuss, Ivan J.; Shigehiro, Tsukasa; Kawaki, Harumi; Mizuno-Kamiya, Masako; Kondoh, Nobuo; Seno, Masaharu.

In: Oncology Reports, Vol. 38, No. 1, 2017, p. 449-455.

Research output: Contribution to journalArticle

Masuda, J, Takayama, E, Strober, W, Satoh, A, Morimoto, Y, Honjo, Y, Ichinohe, T, Tokuno, SI, Ishizuka, T, Nakata, T, Mizutani, A, Umemura, N, Kitani, A, Fuss, IJ, Shigehiro, T, Kawaki, H, Mizuno-Kamiya, M, Kondoh, N & Seno, M 2017, 'Tumor growth limited to subcutaneous site vs tumor growth in pulmonary site exhibit differential effects on systemic immunities', Oncology Reports, vol. 38, no. 1, pp. 449-455. https://doi.org/10.3892/or.2017.5646
Masuda, Junko ; Takayama, Eiji ; Strober, Warren ; Satoh, Ayano ; Morimoto, Yuji ; Honjo, Yasuko ; Ichinohe, Tatsuo ; Tokuno, Shin Ichi ; Ishizuka, Toshiaki ; Nakata, Takahiro ; Mizutani, Akifumi ; Umemura, Naoki ; Kitani, Atsushi ; Fuss, Ivan J. ; Shigehiro, Tsukasa ; Kawaki, Harumi ; Mizuno-Kamiya, Masako ; Kondoh, Nobuo ; Seno, Masaharu. / Tumor growth limited to subcutaneous site vs tumor growth in pulmonary site exhibit differential effects on systemic immunities. In: Oncology Reports. 2017 ; Vol. 38, No. 1. pp. 449-455.
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abstract = "To evaluate systemic immunity associated with tumor growth limited to a subcutaneous site versus growth proceeding at multiple tumor sites, we established syngeneic mouse subcutaneous and pulmonary tumor models by local subcutaneous and intravenous injection of colon carcinoma CT26 cells. We found that splenic myeloid-derived suppressor cell (MDSC) levels were significantly increased in the subcutaneous tumor model but not in the pulmonary tumor model. Furthermore, both CD4+ and CD8+ T cells as well as CD4+ Foxp3+ T cells were significantly decreased in the subcutaneous tumor model and were largely unchanged in the pulmonary tumor model. In addition, the subcutaneous model, but not the pulmonary model, displayed a Th1 polarization bias. This bias was characterized by decreased IL-4, IL-9, and IL-10 production, whereas the pulmonary model displayed increased production of IL-10. These results suggest that the mode of tumor development has differential effects on systemic immunity that may, in turn, influence approaches to treatment of cancer patients.",
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