Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway

Daisuke Yamada, Kohichi Kawahara, Masanobu Ozaki, Takehiko Maeda

Research output: Contribution to journalArticle

3 Citations (Scopus)


We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.

Original languageEnglish
Pages (from-to)942-944
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - Jan 1 2016
Externally publishedYes



  • MTORC1
  • Osteoblast
  • Semaphorin-3a

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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