Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway

Daisuke Yamada; Kohichi Kawahara; Masanobu Ozaki; Takehiko Maeda

doi:10.1080/09168451.2015.1136881

Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway

Daisuke Yamada, Kohichi Kawahara, Masanobu Ozaki, Takehiko Maeda

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.

Original language	English
Pages (from-to)	942-944
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	80
Issue number	5
DOIs	https://doi.org/10.1080/09168451.2015.1136881
Publication status	Published - 2016
Externally published	Yes

Keywords

MTORC1
Osteoblast
Semaphorin-3a

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2015.1136881

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title = "Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway",

abstract = "We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.",

keywords = "MTORC1, Osteoblast, Semaphorin-3a",

author = "Daisuke Yamada and Kohichi Kawahara and Masanobu Ozaki and Takehiko Maeda",

note = "Publisher Copyright: {\textcopyright} 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry.",

year = "2016",

doi = "10.1080/09168451.2015.1136881",

language = "English",

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T1 - Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway

AU - Yamada, Daisuke

AU - Kawahara, Kohichi

AU - Ozaki, Masanobu

AU - Maeda, Takehiko

PY - 2016

Y1 - 2016

N2 - We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.

AB - We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.

KW - MTORC1

KW - Osteoblast

KW - Semaphorin-3a

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ER -

Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway

Abstract

Keywords

ASJC Scopus subject areas

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