Trophic effect of insulin-like growth factor-I on metanephric development

Relationship to proteoglycans

Z. Z. Liu, A. Kumar, E. I. Wallner, Jun Wada, F. A. Carone, Y. S. Kanwar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and correlated with phenotypic and gene expression and synthesis of extracellular matrix (ECM) proteoglycans (PGs). Antisense experiments were carried out to elucidate the role of endogenous (IGF-I in metanephric development. Mouse metanephroi, harvested at 13th day of gestation, were exposed to IGF-I (100 ng/ml) in an organ culture for 7 days. An enlargement of the metanephroi with accentuation of its lobules, and increase in the nephron population and [3H]thymidine incorporation was observed. Immunofluorescence studies and Southern blot analysis of polymerase chain reaction products indicated augmented expression of the ECM PGs. A heavy concentration of [35S]sulfate-associated radioactivity over the tips of ureteric bud branches and ECM components of maturing glomeruli was seen. Maximal effect of radioincorporation was observed on day-4 of the culture, the period when the concentration of endogenous IGF-I is the highest. PGs synthesized had elevated proportions of chondroitin sulfate vs heparan sulfate and of free chains, and reduced charge-density characteristics. Immunoprecipitation studies of [35S]methionine-labeled glycoproteins revealed an increased synthesis of core-peptide of the PGs. IGE-I antisense oligonucleotide caused retardation in the growth of the kidneys along with the decrease in de novo synthesis of PGs. These findings indicate that IGF-I, a polypeptide essential to the renal growth and development, has a trophic effect on the embryonic kidney during the postinductive period of metanephric development, and the observed response has a temporal relationship with the increased synthesis of the PGs.

Original languageEnglish
Pages (from-to)378-391
Number of pages14
JournalEuropean Journal of Cell Biology
Volume65
Issue number2
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Proteoglycans
Insulin-Like Growth Factor I
Extracellular Matrix
Kidney
Growth and Development
Peptides
Heparitin Sulfate
Chondroitin Sulfates
Antisense Oligonucleotides
Organ Culture Techniques
Nephrons
Southern Blotting
Immunoprecipitation
Methionine
Thymidine
Radioactivity
Sulfates
Fluorescent Antibody Technique
Glycoproteins
Hormones

Keywords

  • Antisense-oligonucleotide
  • Extracellular matrix
  • Insulin-like growth factor-I
  • Metanephric development
  • Proteoglycans

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Liu, Z. Z., Kumar, A., Wallner, E. I., Wada, J., Carone, F. A., & Kanwar, Y. S. (1994). Trophic effect of insulin-like growth factor-I on metanephric development: Relationship to proteoglycans. European Journal of Cell Biology, 65(2), 378-391.

Trophic effect of insulin-like growth factor-I on metanephric development : Relationship to proteoglycans. / Liu, Z. Z.; Kumar, A.; Wallner, E. I.; Wada, Jun; Carone, F. A.; Kanwar, Y. S.

In: European Journal of Cell Biology, Vol. 65, No. 2, 1994, p. 378-391.

Research output: Contribution to journalArticle

Liu, Z. Z. ; Kumar, A. ; Wallner, E. I. ; Wada, Jun ; Carone, F. A. ; Kanwar, Y. S. / Trophic effect of insulin-like growth factor-I on metanephric development : Relationship to proteoglycans. In: European Journal of Cell Biology. 1994 ; Vol. 65, No. 2. pp. 378-391.
@article{560576c9daf14065a57128586f76f7fd,
title = "Trophic effect of insulin-like growth factor-I on metanephric development: Relationship to proteoglycans",
abstract = "Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and correlated with phenotypic and gene expression and synthesis of extracellular matrix (ECM) proteoglycans (PGs). Antisense experiments were carried out to elucidate the role of endogenous (IGF-I in metanephric development. Mouse metanephroi, harvested at 13th day of gestation, were exposed to IGF-I (100 ng/ml) in an organ culture for 7 days. An enlargement of the metanephroi with accentuation of its lobules, and increase in the nephron population and [3H]thymidine incorporation was observed. Immunofluorescence studies and Southern blot analysis of polymerase chain reaction products indicated augmented expression of the ECM PGs. A heavy concentration of [35S]sulfate-associated radioactivity over the tips of ureteric bud branches and ECM components of maturing glomeruli was seen. Maximal effect of radioincorporation was observed on day-4 of the culture, the period when the concentration of endogenous IGF-I is the highest. PGs synthesized had elevated proportions of chondroitin sulfate vs heparan sulfate and of free chains, and reduced charge-density characteristics. Immunoprecipitation studies of [35S]methionine-labeled glycoproteins revealed an increased synthesis of core-peptide of the PGs. IGE-I antisense oligonucleotide caused retardation in the growth of the kidneys along with the decrease in de novo synthesis of PGs. These findings indicate that IGF-I, a polypeptide essential to the renal growth and development, has a trophic effect on the embryonic kidney during the postinductive period of metanephric development, and the observed response has a temporal relationship with the increased synthesis of the PGs.",
keywords = "Antisense-oligonucleotide, Extracellular matrix, Insulin-like growth factor-I, Metanephric development, Proteoglycans",
author = "Liu, {Z. Z.} and A. Kumar and Wallner, {E. I.} and Jun Wada and Carone, {F. A.} and Kanwar, {Y. S.}",
year = "1994",
language = "English",
volume = "65",
pages = "378--391",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - Trophic effect of insulin-like growth factor-I on metanephric development

T2 - Relationship to proteoglycans

AU - Liu, Z. Z.

AU - Kumar, A.

AU - Wallner, E. I.

AU - Wada, Jun

AU - Carone, F. A.

AU - Kanwar, Y. S.

PY - 1994

Y1 - 1994

N2 - Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and correlated with phenotypic and gene expression and synthesis of extracellular matrix (ECM) proteoglycans (PGs). Antisense experiments were carried out to elucidate the role of endogenous (IGF-I in metanephric development. Mouse metanephroi, harvested at 13th day of gestation, were exposed to IGF-I (100 ng/ml) in an organ culture for 7 days. An enlargement of the metanephroi with accentuation of its lobules, and increase in the nephron population and [3H]thymidine incorporation was observed. Immunofluorescence studies and Southern blot analysis of polymerase chain reaction products indicated augmented expression of the ECM PGs. A heavy concentration of [35S]sulfate-associated radioactivity over the tips of ureteric bud branches and ECM components of maturing glomeruli was seen. Maximal effect of radioincorporation was observed on day-4 of the culture, the period when the concentration of endogenous IGF-I is the highest. PGs synthesized had elevated proportions of chondroitin sulfate vs heparan sulfate and of free chains, and reduced charge-density characteristics. Immunoprecipitation studies of [35S]methionine-labeled glycoproteins revealed an increased synthesis of core-peptide of the PGs. IGE-I antisense oligonucleotide caused retardation in the growth of the kidneys along with the decrease in de novo synthesis of PGs. These findings indicate that IGF-I, a polypeptide essential to the renal growth and development, has a trophic effect on the embryonic kidney during the postinductive period of metanephric development, and the observed response has a temporal relationship with the increased synthesis of the PGs.

AB - Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and correlated with phenotypic and gene expression and synthesis of extracellular matrix (ECM) proteoglycans (PGs). Antisense experiments were carried out to elucidate the role of endogenous (IGF-I in metanephric development. Mouse metanephroi, harvested at 13th day of gestation, were exposed to IGF-I (100 ng/ml) in an organ culture for 7 days. An enlargement of the metanephroi with accentuation of its lobules, and increase in the nephron population and [3H]thymidine incorporation was observed. Immunofluorescence studies and Southern blot analysis of polymerase chain reaction products indicated augmented expression of the ECM PGs. A heavy concentration of [35S]sulfate-associated radioactivity over the tips of ureteric bud branches and ECM components of maturing glomeruli was seen. Maximal effect of radioincorporation was observed on day-4 of the culture, the period when the concentration of endogenous IGF-I is the highest. PGs synthesized had elevated proportions of chondroitin sulfate vs heparan sulfate and of free chains, and reduced charge-density characteristics. Immunoprecipitation studies of [35S]methionine-labeled glycoproteins revealed an increased synthesis of core-peptide of the PGs. IGE-I antisense oligonucleotide caused retardation in the growth of the kidneys along with the decrease in de novo synthesis of PGs. These findings indicate that IGF-I, a polypeptide essential to the renal growth and development, has a trophic effect on the embryonic kidney during the postinductive period of metanephric development, and the observed response has a temporal relationship with the increased synthesis of the PGs.

KW - Antisense-oligonucleotide

KW - Extracellular matrix

KW - Insulin-like growth factor-I

KW - Metanephric development

KW - Proteoglycans

UR - http://www.scopus.com/inward/record.url?scp=0028598364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028598364&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 378

EP - 391

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 2

ER -