Abstract
A series of solvent mediators containing a phosphoryl (P{double bond, long}O) group, such as tris(2-ethylhexyl)phosphate, bis(2-ethylhexyl) 2-ethylhexylphosphonate, 2-ethylhexyl bis(2-ethylhexyl)phosphinate, and tris(2-ethylhexyl)phosphine oxide, were used to construct serotonin-selective membrane electrodes. We found that replacing the alkoxy groups attached to phosphorus atoms in P{double bond, long}O groups with alkyl groups strengthened the response of the electrode to serotonin, suppressing remarkably interference from inorganic cations, such as Na+. Thus, an electrode combining tris(2-ethylhexyl)phosphine oxide with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate, gave a detection limit of 9 × 10-6 M with a slope of 55.2 mV per concentration decade in physiological saline containing 150 mM NaCl and 10 mM NaH2PO4/Na2HPO4 (pH 7.4). This is the best detection limit of any serotonin-selective electrode developed to date. The selectivity of this electrode for serotonin was over 103 times that for inorganic cations, such as Na+ and K+, and lipophilic quaternary ammonium ions, such as acetylcholine and (C2H5)4N+. Using the electrode, we measured the amount of serotonin released from platelets and found that the results agreed well with those obtained by a conventional fluorimetric assay of serotonin.
| Original language | English |
|---|---|
| Pages (from-to) | 36-41 |
| Number of pages | 6 |
| Journal | Analytica Chimica Acta |
| Volume | 565 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 13 2006 |
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Keywords
- Ion-selective electrode
- Platelet
- Potentiometry
- Serotonin determination
- Solvent mediator
- Tris(2-ethylhexyl)phosphine oxide
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Tris(2-ethylhexyl)phosphine oxide as an effective solvent mediator for constructing a serotonin-selective membrane electrode. / Ueda, Keisuke; Yonemoto, Rei; Komagoe, Keiko; Masuda, Kazufumi; Hanioka, Nobumitsu; Narimatsu, Shizuo; Katsu, Takashi.
In: Analytica Chimica Acta, Vol. 565, No. 1, 13.04.2006, p. 36-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tris(2-ethylhexyl)phosphine oxide as an effective solvent mediator for constructing a serotonin-selective membrane electrode
AU - Ueda, Keisuke
AU - Yonemoto, Rei
AU - Komagoe, Keiko
AU - Masuda, Kazufumi
AU - Hanioka, Nobumitsu
AU - Narimatsu, Shizuo
AU - Katsu, Takashi
PY - 2006/4/13
Y1 - 2006/4/13
N2 - A series of solvent mediators containing a phosphoryl (P{double bond, long}O) group, such as tris(2-ethylhexyl)phosphate, bis(2-ethylhexyl) 2-ethylhexylphosphonate, 2-ethylhexyl bis(2-ethylhexyl)phosphinate, and tris(2-ethylhexyl)phosphine oxide, were used to construct serotonin-selective membrane electrodes. We found that replacing the alkoxy groups attached to phosphorus atoms in P{double bond, long}O groups with alkyl groups strengthened the response of the electrode to serotonin, suppressing remarkably interference from inorganic cations, such as Na+. Thus, an electrode combining tris(2-ethylhexyl)phosphine oxide with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate, gave a detection limit of 9 × 10-6 M with a slope of 55.2 mV per concentration decade in physiological saline containing 150 mM NaCl and 10 mM NaH2PO4/Na2HPO4 (pH 7.4). This is the best detection limit of any serotonin-selective electrode developed to date. The selectivity of this electrode for serotonin was over 103 times that for inorganic cations, such as Na+ and K+, and lipophilic quaternary ammonium ions, such as acetylcholine and (C2H5)4N+. Using the electrode, we measured the amount of serotonin released from platelets and found that the results agreed well with those obtained by a conventional fluorimetric assay of serotonin.
AB - A series of solvent mediators containing a phosphoryl (P{double bond, long}O) group, such as tris(2-ethylhexyl)phosphate, bis(2-ethylhexyl) 2-ethylhexylphosphonate, 2-ethylhexyl bis(2-ethylhexyl)phosphinate, and tris(2-ethylhexyl)phosphine oxide, were used to construct serotonin-selective membrane electrodes. We found that replacing the alkoxy groups attached to phosphorus atoms in P{double bond, long}O groups with alkyl groups strengthened the response of the electrode to serotonin, suppressing remarkably interference from inorganic cations, such as Na+. Thus, an electrode combining tris(2-ethylhexyl)phosphine oxide with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate, gave a detection limit of 9 × 10-6 M with a slope of 55.2 mV per concentration decade in physiological saline containing 150 mM NaCl and 10 mM NaH2PO4/Na2HPO4 (pH 7.4). This is the best detection limit of any serotonin-selective electrode developed to date. The selectivity of this electrode for serotonin was over 103 times that for inorganic cations, such as Na+ and K+, and lipophilic quaternary ammonium ions, such as acetylcholine and (C2H5)4N+. Using the electrode, we measured the amount of serotonin released from platelets and found that the results agreed well with those obtained by a conventional fluorimetric assay of serotonin.
KW - Ion-selective electrode
KW - Platelet
KW - Potentiometry
KW - Serotonin determination
KW - Solvent mediator
KW - Tris(2-ethylhexyl)phosphine oxide
UR - http://www.scopus.com/inward/record.url?scp=33646061944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646061944&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2006.02.001
DO - 10.1016/j.aca.2006.02.001
M3 - Article
AN - SCOPUS:33646061944
VL - 565
SP - 36
EP - 41
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -