Tris(2-ethylhexyl)phosphine oxide as an effective solvent mediator for constructing a serotonin-selective membrane electrode

Keisuke Ueda, Rei Yonemoto, Keiko Komagoe, Kazufumi Masuda, Nobumitsu Hanioka, Shizuo Narimatsu, Takashi Katsu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A series of solvent mediators containing a phosphoryl (P{double bond, long}O) group, such as tris(2-ethylhexyl)phosphate, bis(2-ethylhexyl) 2-ethylhexylphosphonate, 2-ethylhexyl bis(2-ethylhexyl)phosphinate, and tris(2-ethylhexyl)phosphine oxide, were used to construct serotonin-selective membrane electrodes. We found that replacing the alkoxy groups attached to phosphorus atoms in P{double bond, long}O groups with alkyl groups strengthened the response of the electrode to serotonin, suppressing remarkably interference from inorganic cations, such as Na+. Thus, an electrode combining tris(2-ethylhexyl)phosphine oxide with an ion-exchanger, sodium tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate, gave a detection limit of 9 × 10-6 M with a slope of 55.2 mV per concentration decade in physiological saline containing 150 mM NaCl and 10 mM NaH2PO4/Na2HPO4 (pH 7.4). This is the best detection limit of any serotonin-selective electrode developed to date. The selectivity of this electrode for serotonin was over 103 times that for inorganic cations, such as Na+ and K+, and lipophilic quaternary ammonium ions, such as acetylcholine and (C2H5)4N+. Using the electrode, we measured the amount of serotonin released from platelets and found that the results agreed well with those obtained by a conventional fluorimetric assay of serotonin.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalAnalytica Chimica Acta
Volume565
Issue number1
DOIs
Publication statusPublished - Apr 13 2006

Fingerprint

phosphine
Oxides
Serotonin
Electrodes
electrode
oxide
membrane
Membranes
Limit of Detection
Cations
cation
Ions
Ion exchangers
borate
Platelets
Ammonium Compounds
Phosphorus
Acetylcholine
Assays
Blood Platelets

Keywords

  • Ion-selective electrode
  • Platelet
  • Potentiometry
  • Serotonin determination
  • Solvent mediator
  • Tris(2-ethylhexyl)phosphine oxide

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Tris(2-ethylhexyl)phosphine oxide as an effective solvent mediator for constructing a serotonin-selective membrane electrode. / Ueda, Keisuke; Yonemoto, Rei; Komagoe, Keiko; Masuda, Kazufumi; Hanioka, Nobumitsu; Narimatsu, Shizuo; Katsu, Takashi.

In: Analytica Chimica Acta, Vol. 565, No. 1, 13.04.2006, p. 36-41.

Research output: Contribution to journalArticle

Ueda, Keisuke ; Yonemoto, Rei ; Komagoe, Keiko ; Masuda, Kazufumi ; Hanioka, Nobumitsu ; Narimatsu, Shizuo ; Katsu, Takashi. / Tris(2-ethylhexyl)phosphine oxide as an effective solvent mediator for constructing a serotonin-selective membrane electrode. In: Analytica Chimica Acta. 2006 ; Vol. 565, No. 1. pp. 36-41.
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