Triiodothyronine (T3) Enhances the Stimulatory Effect of 1, 25-Dihydroxyvitamin D3 on Calbindin-D28K mRNA Expression in the Kidney and Intestine but Not in Cerebellum of the Chick

A. Sechman, K. Shimada, N. Saito, T. Ieda, T. Ono

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The present study was conducted to investigate the role of thyroid hormones in the regulation of gene expression of calbindin-D28k (CaBP-D28K) in the chicken. By employing slot blot and RIA analyses, levels of CaBP-D28K mRNA and CaBP-D28K protein in the intestine, kidney, cerebellum and liver were measured 6 and 12 h after i.m. injection of 1, 25-dihydroxyvitamin D3 [1, 25 (OH)2D3; 250 ng/chick] and 3, 5, 3′-triiodothyronine (T3; 500 ng/chick) in one-day-old chicks. The abundant messages of CaBP-D28K mRNA were detected in the intestine, kidney and cerebellum while there was little message in the liver. After 1, 25 (OH)2D3 treatment (6 + 12 hours), levels of CaBP-D28K mRNA increased in the intestine, but there was no change in the mRNA levels in the kidney and cerebellum. Although T3 alone had no effect on CaBP-D28K mRNA levels, simultaneous administration of T3 enhanced the. 1, 25 (OH)2D3 effect on levels of CaBP-D28K mRNA in the intestine both 6 and 12 h post-treatment, and in the kidney 12 h post-treatment. At a protein level, co-treatment with 1, 25 (OH)2D3 and T3 elicited a significant increase in CaBP-D28K expression in the intestine 12 h post-treatment, as compared to treatment with only 1, 25 (OH)2D3, whereas no differences were observed in the CaBP-D28K protein levels in the kidney and cerebellum. These results suggest that thyroid hormones may play a synergistic role with 1, 25 (OH)2D3 for CaBP-D28K gene expression in the intestine and kidney in chicks.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalAsian-Australasian Journal of Animal Sciences
Volume9
Issue number1
DOIs
Publication statusPublished - Feb 1996
Externally publishedYes

Keywords

  • 1, 25-dihydroxycholecalciferol
  • Calbindin-D
  • Chick
  • Gene Expression
  • Triiodothyronine (T)

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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