Triclosan, an antibacterial agent, increases intracellular Zn 2+ concentration in rat thymocytes: Its relation to oxidative stress

Ikumi Tamura, Yasuhiro Kanbara, Minoru Saito, Kanna Horimoto, Masaya Satoh, Hiroshi Yamamoto, Yasuo Oyama

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Triclosan is used as an antibacterial agent in household items and personal care products. Since this compound is found in maternal milk of humans and bodies of wild animals, there is growing concern among some consumer groups and scientific community that triclosan is adverse for humans and wild animals. In order to estimate adverse actions of triclosan, the effects of triclosan on intracellular Zn 2+ concentration and cellular thiol content were studied in rat thymocytes by the use of flow cytometer with appropriate fluorescent probes. Triclosan at 1-3μM (sublethal concentrations) increased the intensity of FluoZin-3 fluorescence (intracellular Zn 2+ concentration) and decreased the intensity of 5-chloromethylfluorescein (5-CMF) fluorescence (cellular thiol content). Negative correlation (r=-0.985) between triclosan-induced changes in FluoZin-3 and 5-CMF fluorescences was found. Removal of external Zn 2+ did not significantly affect the triclosan-induced augmentation of FluoZin-3 fluorescence, suggesting an intracellular Zn 2+ release by triclosan. These actions of triclosan were similar to those of H 2O 2 and triclosan significantly potentiated the cytotoxicity of H 2O 2. Therefore, the results may suggest that triclosan at sublethal concentrations induces oxidative stress that decreases cellular thiol content, resulting in an increase in intracellular Zn 2+ concentration by Zn 2+ release from intracellular store(s). Since recent studies show many physiological roles of intracellular Zn 2+ in cellular functions, the triclosan-induced disturbance of cellular Zn 2+ homeostasis may induce adverse actions on the cells.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalChemosphere
Volume86
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Fingerprint

Triclosan
Bactericides
Oxidative stress
Thymocytes
Rats
thiol
Oxidative Stress
fluorescence
Fluorescence
Anti-Bacterial Agents
Animals
homeostasis
Cytotoxicity
Sulfhydryl Compounds
milk
Wild Animals
probe
disturbance
oxidative stress
Fluorescent Dyes

Keywords

  • Antibacterial agent
  • Intracellular Zn
  • Oxidative stress
  • Thiols
  • Triclosan

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Triclosan, an antibacterial agent, increases intracellular Zn 2+ concentration in rat thymocytes : Its relation to oxidative stress. / Tamura, Ikumi; Kanbara, Yasuhiro; Saito, Minoru; Horimoto, Kanna; Satoh, Masaya; Yamamoto, Hiroshi; Oyama, Yasuo.

In: Chemosphere, Vol. 86, No. 1, 01.2012, p. 70-75.

Research output: Contribution to journalArticle

Tamura, Ikumi ; Kanbara, Yasuhiro ; Saito, Minoru ; Horimoto, Kanna ; Satoh, Masaya ; Yamamoto, Hiroshi ; Oyama, Yasuo. / Triclosan, an antibacterial agent, increases intracellular Zn 2+ concentration in rat thymocytes : Its relation to oxidative stress. In: Chemosphere. 2012 ; Vol. 86, No. 1. pp. 70-75.
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