Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

Yo Sonoda, Alex Cameron, Simon Newstead, Hiroshi Omote, Yoshinori Moriyama, Michihiro Kasahara, So Iwata, David Drew

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.

Original languageEnglish
Pages (from-to)2539-2547
Number of pages9
JournalFEBS Letters
Volume584
Issue number12
DOIs
Publication statusPublished - Jun 2010

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Membrane Proteins
X-Rays
X rays
Proteins

Keywords

  • Fluorescence-detection size-exclusion chromatography
  • Green Fluorescent Protein
  • Mammalian transporter
  • Membrane protein
  • X-ray structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Sonoda, Y., Cameron, A., Newstead, S., Omote, H., Moriyama, Y., Kasahara, M., ... Drew, D. (2010). Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS Letters, 584(12), 2539-2547. https://doi.org/10.1016/j.febslet.2010.04.015

Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. / Sonoda, Yo; Cameron, Alex; Newstead, Simon; Omote, Hiroshi; Moriyama, Yoshinori; Kasahara, Michihiro; Iwata, So; Drew, David.

In: FEBS Letters, Vol. 584, No. 12, 06.2010, p. 2539-2547.

Research output: Contribution to journalArticle

Sonoda, Y, Cameron, A, Newstead, S, Omote, H, Moriyama, Y, Kasahara, M, Iwata, S & Drew, D 2010, 'Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins', FEBS Letters, vol. 584, no. 12, pp. 2539-2547. https://doi.org/10.1016/j.febslet.2010.04.015
Sonoda, Yo ; Cameron, Alex ; Newstead, Simon ; Omote, Hiroshi ; Moriyama, Yoshinori ; Kasahara, Michihiro ; Iwata, So ; Drew, David. / Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. In: FEBS Letters. 2010 ; Vol. 584, No. 12. pp. 2539-2547.
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