Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

Yo Sonoda; Alex Cameron; Simon Newstead; Hiroshi Omote; Yoshinori Moriyama; Michihiro Kasahara; So Iwata; David Drew

doi:10.1016/j.febslet.2010.04.015

Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

Yo Sonoda, Alex Cameron, Simon Newstead, Hiroshi Omote, Yoshinori Moriyama, Michihiro Kasahara, So Iwata, David Drew

Research output: Contribution to journal › Review article › peer-review

39 Citations (Scopus)

Abstract

The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.

Original language	English
Pages (from-to)	2539-2547
Number of pages	9
Journal	FEBS Letters
Volume	584
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2010.04.015
Publication status	Published - Jun 2010

Keywords

Fluorescence-detection size-exclusion chromatography
Green Fluorescent Protein
Mammalian transporter
Membrane protein
X-ray structure

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2010.04.015

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abstract = "The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.",

keywords = "Fluorescence-detection size-exclusion chromatography, Green Fluorescent Protein, Mammalian transporter, Membrane protein, X-ray structure",

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AU - Cameron, Alex

AU - Newstead, Simon

AU - Omote, Hiroshi

AU - Moriyama, Yoshinori

AU - Kasahara, Michihiro

AU - Iwata, So

AU - Drew, David

PY - 2010/6

Y1 - 2010/6

N2 - The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.

AB - The rate at which X-ray structures of membrane proteins are solved is on a par with that of soluble proteins in the late 1970s. There are still many obstacles facing the membrane protein structural community. Recently, there have been several technical achievements in the field that have started to dramatically accelerate structural studies. Here, we summarize these so-called 'tricks-of-the-trade' and include case studies of several mammalian transporters.

KW - Fluorescence-detection size-exclusion chromatography

KW - Green Fluorescent Protein

KW - Mammalian transporter

KW - Membrane protein

KW - X-ray structure

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Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins

Abstract

Keywords

ASJC Scopus subject areas

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