TY - JOUR
T1 - Trichostatin A-treated eight-cell bovine embryos had increased histone acetylation and gene expression, with increased cell numbers at the blastocyst stage
AU - Shiratsuki, Shogo
AU - Iwata, Hisataka
AU - Kimura, Koji
AU - Kuge, Takeshi
AU - Monji, Yasunori
AU - Kuwayama, Takehito
N1 - Funding Information:
This study was supported by the Promotion and Mutual Aid Corporation for Private Schools of Japan.
PY - 2011/3/15
Y1 - 2011/3/15
N2 - The objective was to determine the effects of trichostatin A (TSA), a potent histone deacetylase inhibitor, on eight-cell bovine embryos. That treatment increased histone acetylation was confirmed by immunostaining with anti-AcH4K5 and AcH4K8 antibodies. Embryos treated with TSA (100 nM) for various intervals (4, 8, and 12 h) developed to the blastocyst stage as frequently as untreated embryos (average development rate, 49.5%). Treatment with TSA for 12 h increased (P < 0.05) the numbers of inner cell mass (ICM) cells and total cells (TC), as well as the ICM/TC ratio in the blastocyst, but the number of cells in the trophectoderm decreased (P < 0.05). Treated embryos had increased relative abundance (RA) of OCT3/4 and E-CADHERIN mRNA relative to controls at the morula stage (P < 0.05), however, the RA of CDX2 mRNA was unchanged. In conclusion, TSA-treated eight-cell stage embryos had increased histone acetylation and gene expression, which increased ICM and TC numbers and the ICM/TC ratio, but significantly decreased the number of cells in the trophectoderm of resulting blastocysts.
AB - The objective was to determine the effects of trichostatin A (TSA), a potent histone deacetylase inhibitor, on eight-cell bovine embryos. That treatment increased histone acetylation was confirmed by immunostaining with anti-AcH4K5 and AcH4K8 antibodies. Embryos treated with TSA (100 nM) for various intervals (4, 8, and 12 h) developed to the blastocyst stage as frequently as untreated embryos (average development rate, 49.5%). Treatment with TSA for 12 h increased (P < 0.05) the numbers of inner cell mass (ICM) cells and total cells (TC), as well as the ICM/TC ratio in the blastocyst, but the number of cells in the trophectoderm decreased (P < 0.05). Treated embryos had increased relative abundance (RA) of OCT3/4 and E-CADHERIN mRNA relative to controls at the morula stage (P < 0.05), however, the RA of CDX2 mRNA was unchanged. In conclusion, TSA-treated eight-cell stage embryos had increased histone acetylation and gene expression, which increased ICM and TC numbers and the ICM/TC ratio, but significantly decreased the number of cells in the trophectoderm of resulting blastocysts.
KW - Blastocyst
KW - Bovine embryo development
KW - Gene expression
KW - Trichostatin A
UR - http://www.scopus.com/inward/record.url?scp=79951810101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951810101&partnerID=8YFLogxK
U2 - 10.1016/j.theriogenology.2010.10.026
DO - 10.1016/j.theriogenology.2010.10.026
M3 - Article
C2 - 21144563
AN - SCOPUS:79951810101
VL - 75
SP - 841
EP - 848
JO - Theriogenology
JF - Theriogenology
SN - 0093-691X
IS - 5
ER -