Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes

Takeshi Hano, Yuji Oshima, Sang Gyoon Kim, Hina Satone, Yumi Oba, Takeshi Kitano, Suguru Inoue, Yohei Shimasaki, Tsuneo Honjo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

Original languageEnglish
Pages (from-to)927-933
Number of pages7
JournalChemosphere
Volume69
Issue number6
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

Oryzias
tributyltin
embryonic development
Embryonic Development
embryo
Genes
Sex Differentiation
hatching
masculinization
egg
Ovum
Fertilization
Sex Characteristics
mortality
Embryonic Structures
gene
Mortality

Keywords

  • Endocrine disruption
  • Medaka
  • Nanoinjection
  • Tributyltin

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Hano, T., Oshima, Y., Kim, S. G., Satone, H., Oba, Y., Kitano, T., ... Honjo, T. (2007). Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes. Chemosphere, 69(6), 927-933. https://doi.org/10.1016/j.chemosphere.2007.05.093

Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes. / Hano, Takeshi; Oshima, Yuji; Kim, Sang Gyoon; Satone, Hina; Oba, Yumi; Kitano, Takeshi; Inoue, Suguru; Shimasaki, Yohei; Honjo, Tsuneo.

In: Chemosphere, Vol. 69, No. 6, 10.2007, p. 927-933.

Research output: Contribution to journalArticle

Hano, T, Oshima, Y, Kim, SG, Satone, H, Oba, Y, Kitano, T, Inoue, S, Shimasaki, Y & Honjo, T 2007, 'Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes', Chemosphere, vol. 69, no. 6, pp. 927-933. https://doi.org/10.1016/j.chemosphere.2007.05.093
Hano, Takeshi ; Oshima, Yuji ; Kim, Sang Gyoon ; Satone, Hina ; Oba, Yumi ; Kitano, Takeshi ; Inoue, Suguru ; Shimasaki, Yohei ; Honjo, Tsuneo. / Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes. In: Chemosphere. 2007 ; Vol. 69, No. 6. pp. 927-933.
@article{a4133485cfd044acb3a5b2c72c26cef7,
title = "Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes",
abstract = "We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).",
keywords = "Endocrine disruption, Medaka, Nanoinjection, Tributyltin",
author = "Takeshi Hano and Yuji Oshima and Kim, {Sang Gyoon} and Hina Satone and Yumi Oba and Takeshi Kitano and Suguru Inoue and Yohei Shimasaki and Tsuneo Honjo",
year = "2007",
month = "10",
doi = "10.1016/j.chemosphere.2007.05.093",
language = "English",
volume = "69",
pages = "927--933",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Tributyltin causes abnormal development in embryos of medaka, Oryzias latipes

AU - Hano, Takeshi

AU - Oshima, Yuji

AU - Kim, Sang Gyoon

AU - Satone, Hina

AU - Oba, Yumi

AU - Kitano, Takeshi

AU - Inoue, Suguru

AU - Shimasaki, Yohei

AU - Honjo, Tsuneo

PY - 2007/10

Y1 - 2007/10

N2 - We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

AB - We examined the effects of tributyltin (TBT) on embryonic development, hatching success and sexual differentiation in Japanese medaka (Oryzias latipes). Embryos (within 8 h after fertilization) were exposed to TBT in ovo via nanoinjection at concentrations of 0 (control), 0.16, 0.80, 3.96, 19.2 and 82.1 ng/egg. Embryonic survival, development and hatching were observed. Hatched fry were reared until 60 days when they sexually matured, and sexual differentiation was also examined by accordance of genetic and phenotypic sex, based on existence of DMY (a male determining gene in medaka) and secondary sex characteristics. As results, TBT caused a concentration-dependent mortality and impaired the embryonic development. However, no masculinization was detected at 60 dph medaka adults. Lowest observed effective concentration for inducing abnormal embryonic development was estimated to 0.16 ng/egg (ca. 160 ng/g egg).

KW - Endocrine disruption

KW - Medaka

KW - Nanoinjection

KW - Tributyltin

UR - http://www.scopus.com/inward/record.url?scp=34548609048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548609048&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2007.05.093

DO - 10.1016/j.chemosphere.2007.05.093

M3 - Article

VL - 69

SP - 927

EP - 933

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

IS - 6

ER -