Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus

Hina Satone, Yuji Oshima, Yohei Shimasaki, Takahiko Tawaratsumida, Yumi Oba, Eriko Takahashi, Takeshi Kitano, Shun ichiro Kawabata, Yoshimitsu Kakuta, Tsuneo Honjo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Tributyltin-binding protein type 1 (TBT-bp1) is a newly discovered protein that binds with TBT in the blood of the Japanese flounder, Paralichthys olivaceus. We determined the genomic sequence of TBT-bp1 and found that this protein has a conserved exon-intron structure that is common to the lipocalin protein family. The secondary and tertiary structures of TBT-bp1, predicted from amino acid sequence, included at least two α-helices and eight β-sheets that are conserved in all lipocalins and form a barrel structure that may bind with ligands. Analysis of the gene structure, secondary structure, and tertiary structure demonstrated that TBT-bp1 could be classified as a lipocalin. A homology search revealed the presence of TBT-bp1-like proteins in eight species of teleost. When flounder were injected intraperitoneally with TBT-d27 at 11.6 μg/fish, TBT-d27 was detected in the blood and in the skin mucus. The concentration of TBT-d27 in mucus was approximately 1/100 of that in the serum. Western blotting analysis revealed that TBT-bp1 was present in the skin mucus. These results suggest that TBT-bp1 in Japanese flounder binds with TBT and is excreted from the body via the mucus.

Original languageEnglish
Pages (from-to)292-299
Number of pages8
JournalAquatic Toxicology
Volume90
Issue number4
DOIs
Publication statusPublished - Dec 11 2008

Keywords

  • Excretion
  • Lipocalin
  • Skin mucus
  • Tributyltin
  • Tributyltin-binding protein

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus'. Together they form a unique fingerprint.

  • Cite this

    Satone, H., Oshima, Y., Shimasaki, Y., Tawaratsumida, T., Oba, Y., Takahashi, E., Kitano, T., Kawabata, S. I., Kakuta, Y., & Honjo, T. (2008). Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus. Aquatic Toxicology, 90(4), 292-299. https://doi.org/10.1016/j.aquatox.2008.08.019