Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

Naoya Kobayashi, Masahiro Miyazaki, Kenichi Fukaya, Yusuke Inoue, Masakiyo Sakaguchi, Hirofumi Noguchi, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Noriaki Tanaka, Masayoshi Namba

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22 Citations (Scopus)

Abstract

Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

Original languageEnglish
Pages (from-to)733-735
Number of pages3
JournalCell Transplantation
Volume9
Issue number5
DOIs
Publication statusPublished - 2000

Keywords

  • Acute liver failure
  • Hepatocyte transplantation
  • Immoralized human hepatocytes
  • Simian virus 40 large T antigen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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    Kobayashi, N., Miyazaki, M., Fukaya, K., Inoue, Y., Sakaguchi, M., Noguchi, H., Matsumura, T., Watanabe, T., Totsugawa, T., Tanaka, N., & Namba, M. (2000). Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes. Cell Transplantation, 9(5), 733-735. https://doi.org/10.1177/096368970000900524