Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes

Naoya Kobayashi, Masahiro Miyazaki, Kenichi Fukaya, Yusuke Inoue, Masakiyo Sakaguchi, Hirofumi Noguchi, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Noriaki Tanaka, Masayoshi Namba

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

Original languageEnglish
Pages (from-to)733-735
Number of pages3
JournalCell Transplantation
Volume9
Issue number5
Publication statusPublished - 2000

Fingerprint

Acute Liver Failure
Liver
Hepatocytes
Transplantation
Hyperammonemia
Antigens
Viruses
Simian virus 40
Viral Tumor Antigens
Rats
Cells
Availability
Cell Line
Survival

Keywords

  • Acute liver failure
  • Hepatocyte transplantation
  • Immoralized human hepatocytes
  • Simian virus 40 large T antigen

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes. / Kobayashi, Naoya; Miyazaki, Masahiro; Fukaya, Kenichi; Inoue, Yusuke; Sakaguchi, Masakiyo; Noguchi, Hirofumi; Matsumura, Toshihisa; Watanabe, Takamasa; Totsugawa, Toshinori; Tanaka, Noriaki; Namba, Masayoshi.

In: Cell Transplantation, Vol. 9, No. 5, 2000, p. 733-735.

Research output: Contribution to journalArticle

Kobayashi, N, Miyazaki, M, Fukaya, K, Inoue, Y, Sakaguchi, M, Noguchi, H, Matsumura, T, Watanabe, T, Totsugawa, T, Tanaka, N & Namba, M 2000, 'Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes', Cell Transplantation, vol. 9, no. 5, pp. 733-735.
Kobayashi, Naoya ; Miyazaki, Masahiro ; Fukaya, Kenichi ; Inoue, Yusuke ; Sakaguchi, Masakiyo ; Noguchi, Hirofumi ; Matsumura, Toshihisa ; Watanabe, Takamasa ; Totsugawa, Toshinori ; Tanaka, Noriaki ; Namba, Masayoshi. / Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes. In: Cell Transplantation. 2000 ; Vol. 9, No. 5. pp. 733-735.
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