Treatment of Leptothrix cells with ultrapure water poses a threat to their viability

Tatsuki Kunoh, Tomoko Suzuki, Tomonori Shiraishi, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The genus Leptothrix, a type of Fe/Mn-oxidizing bacteria, is characterized by its formation of an extracellular and microtubular sheath. Although almost all sheaths harvested from natural aquatic environments are hollow, a few chained bacterial cells are occasionally seen within some sheaths of young stage. We previously reported that sheaths of Leptothrix sp. strain OUMS1 cultured in artificial media became hollow with aging due to spontaneous autolysis within the sheaths. In this study, we investigated environmental conditions that lead the OUMS1 cells to die. Treatment of the cells with ultrapure water or acidic buffers (pH 6.0) caused autolysis of the cells. Under these conditions, the plasma membrane and cytoplasm of cells were drastically damaged, resulting in leakage of intracellular electrolytes and relaxation of genomic DNA. The autolysis was suppressed by the presence of Ca2+. The hydrolysis of peptidoglycan by the lysozyme treatment similarly caused autolysis of the cells and was suppressed also by the presence of Ca2+. However, it remains unclear whether the acidic pH-dependent autolysis is attributable to damage of peptidoglycan. It was observed that L. discophora strain SP-6 cells also underwent autolysis when suspended in ultrapure water; it is however, uncertain whether this phenomenon is common among other members of the genus Leptothrix.

Original languageEnglish
Pages (from-to)50-66
Number of pages17
JournalBiology
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 27 2015

Fingerprint

Leptothrix (bacteria)
Leptothrix
Peptidoglycan
Autolysis
autolysis
viability
Water
Cell membranes
Muramidase
Electrolytes
Hydrolysis
Bacteria
Buffers
Aging of materials
water
Cells
cells
peptidoglycans
DNA
calcium

Keywords

  • Acidic pH to cell viability
  • Cell death induced by UPW
  • Damage of bacterial membrane
  • Electrolytes leakage
  • Lack of Ca to cell viability
  • Leptothrix
  • Peptidoglycan
  • Relaxation of DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kunoh, T., Suzuki, T., Shiraishi, T., Kunoh, H., & Takada, J. (2015). Treatment of Leptothrix cells with ultrapure water poses a threat to their viability. Biology, 4(1), 50-66. https://doi.org/10.3390/biology4010050

Treatment of Leptothrix cells with ultrapure water poses a threat to their viability. / Kunoh, Tatsuki; Suzuki, Tomoko; Shiraishi, Tomonori; Kunoh, Hitoshi; Takada, Jun.

In: Biology, Vol. 4, No. 1, 27.01.2015, p. 50-66.

Research output: Contribution to journalArticle

Kunoh, T, Suzuki, T, Shiraishi, T, Kunoh, H & Takada, J 2015, 'Treatment of Leptothrix cells with ultrapure water poses a threat to their viability', Biology, vol. 4, no. 1, pp. 50-66. https://doi.org/10.3390/biology4010050
Kunoh, Tatsuki ; Suzuki, Tomoko ; Shiraishi, Tomonori ; Kunoh, Hitoshi ; Takada, Jun. / Treatment of Leptothrix cells with ultrapure water poses a threat to their viability. In: Biology. 2015 ; Vol. 4, No. 1. pp. 50-66.
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