Transvascular accumulation of sialyl lewis X conjugated liposome in inflamed joints of collagen antibody-induced arthritic (CAIA) mice

Jun Minaguchi, Toshitaka Oohashi, Kiichi Inagawa, Aiji Ohtsuka, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

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Abstract

The aim of the current study was to investigate the specific accumulation of the Sialyl Lewis X (SLX) liposome in inflammation in the collagen-antibody induced arthritic (CAIA) model mice. The SLX-liposome encapsulating fluorescent substance (Cy5.5 or Cy3) was prepared for this study. The SLX-liposome was administered intravenously via the mouse caudal vein. After 1 to 24 h, the accumulation of SLX-liposome was observed using in vivo fluorescent imaging equipment (eXplore Optix), or the knee joints were removed for histological analysis. The in vivo fluorescent imaging showed that the signal was confined to the inflammatory site in the CAIA mice in an inflammatory dependent manner. The signal intensity was stronger at 24 h than at 1 h after injection. In the histological sections, the fluorescent signals were detected in the periarticular soft-tissue, especially in the hyperplastic synovium, including a pannus invasion with inflammatory cells in the CAIA. Intense signals were observed in vessel-like structures 1 h after injection; these were co-labeled with the vascular endothelial cell marker (CD31) and E-selectin, a ligand of the SLX-liposome expressed on activated endothelial cells. The diffused signals from the vessels increased time-dependently at 6 to 24 h after injection. This is the first report to examine the exact localization of the SLX- liposome by encapsulated fluorescence in hyperplastic synovial tissue of CAIA mice. These results suggest the feasibility and potential use of SLX-liposome as a vehicle for the active targeting of drug delivery to inflammatory tissue.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalArchives of Histology and Cytology
Volume71
Issue number3
DOIs
Publication statusPublished - 2008

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Experimental Arthritis
Liposomes
Joints
Antibodies
Injections
Endothelial Cells
E-Selectin
Synovial Membrane
Drug Delivery Systems
Knee Joint
Veins
Fluorescence
Ligands
Inflammation
Equipment and Supplies

ASJC Scopus subject areas

  • Histology

Cite this

Transvascular accumulation of sialyl lewis X conjugated liposome in inflamed joints of collagen antibody-induced arthritic (CAIA) mice. / Minaguchi, Jun; Oohashi, Toshitaka; Inagawa, Kiichi; Ohtsuka, Aiji; Ninomiya, Yoshifumi.

In: Archives of Histology and Cytology, Vol. 71, No. 3, 2008, p. 195-203.

Research output: Contribution to journalArticle

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