Transposases are responsible for the target specificity of IS1397 and ISKpn1 for two different types of palindromic units (PUs)

Caroline Wilde, Frédéric Escartin, Susumu Kokeguchi, Patricia Latour-Lambert, Aude Lectard, Jean Marie Clément

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Insertion sequences (IS)1397 and ISKpn1, found in Escherichia coli and Klebsiella pneumoniae respectively, are IS3 family members that insert specifically into short palindromic repeated sequences (palindromic units or PUs). In this paper, we first show that although PUs are naturally absent from extrachromosomal elements, both ISs are able to transpose from the chromosome or from a plasmid into PUs artificially introduced into target plasmids. We also show that ISKpn1 target specificity is restricted to K.pneumoniae Z1 PU type, whereas IS1397 target specificity is less stringent since the IS fargets the three E.coli Y, Z1 and Z2 PU types indifferently. Experiments of transposition of both ISs driven by both transposases demonstrate that the inverted repeats flanking the ISs are not responsible for this target specificity, which is entirely due to the transposase itself. Implications on ISs evolution are presented.

Original languageEnglish
Pages (from-to)4345-4353
Number of pages9
JournalNucleic acids research
Volume31
Issue number15
DOIs
Publication statusPublished - Aug 1 2003

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Transposases are responsible for the target specificity of IS1397 and ISKpn1 for two different types of palindromic units (PUs)'. Together they form a unique fingerprint.

Cite this