Abstract
To evaluate the role of several specialized mechanisms for D-glucose transport in human oral mucosa, a cultured stratified cell layer derived from human oral mucosa was employed. Although this culture system has been used for reconstructive surgery, we, for the first time, tried to apply this system to the evaluation of nutrients and drug transport. Cell number and transepithelial electrical resistance (TEER) reached steady state 7-8 days after inoculation on the Transwell and TEER values at steady state were 130-140 Ω cm2, which was higher or lower than that of small intestine or Caco-2 cells, respectively. The transport studies were carried out using the cultured epithelium on the Transwell. The transport of D-glucose across the cultured stratified layer of oral epithelial cells was much more extensive than L-glucose, and was inhibited by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, and α-methyl-D-glucoside, a specific substrate of a Na+/glucose cotransporter (SGLT1). The results indicate that the sugar transporters function not only to take up D-glucose by the epithelial cells but also to transport the sugar across the stratified epithelial layer.
| Original language | English |
|---|---|
| Pages (from-to) | 213-219 |
| Number of pages | 7 |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 54 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 |
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ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science
Cite this
Transport of D-glucose across cultured stratified cell layer of human oral mucosal cells. / Kimura, T.; Yamano, H.; Tanaka, A.; Matsumura, T.; Ueda, M.; Ogawara, K. I.; Higaki, Kazutaka.
In: Journal of Pharmacy and Pharmacology, Vol. 54, No. 2, 2002, p. 213-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transport of D-glucose across cultured stratified cell layer of human oral mucosal cells
AU - Kimura, T.
AU - Yamano, H.
AU - Tanaka, A.
AU - Matsumura, T.
AU - Ueda, M.
AU - Ogawara, K. I.
AU - Higaki, Kazutaka
PY - 2002
Y1 - 2002
N2 - To evaluate the role of several specialized mechanisms for D-glucose transport in human oral mucosa, a cultured stratified cell layer derived from human oral mucosa was employed. Although this culture system has been used for reconstructive surgery, we, for the first time, tried to apply this system to the evaluation of nutrients and drug transport. Cell number and transepithelial electrical resistance (TEER) reached steady state 7-8 days after inoculation on the Transwell and TEER values at steady state were 130-140 Ω cm2, which was higher or lower than that of small intestine or Caco-2 cells, respectively. The transport studies were carried out using the cultured epithelium on the Transwell. The transport of D-glucose across the cultured stratified layer of oral epithelial cells was much more extensive than L-glucose, and was inhibited by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, and α-methyl-D-glucoside, a specific substrate of a Na+/glucose cotransporter (SGLT1). The results indicate that the sugar transporters function not only to take up D-glucose by the epithelial cells but also to transport the sugar across the stratified epithelial layer.
AB - To evaluate the role of several specialized mechanisms for D-glucose transport in human oral mucosa, a cultured stratified cell layer derived from human oral mucosa was employed. Although this culture system has been used for reconstructive surgery, we, for the first time, tried to apply this system to the evaluation of nutrients and drug transport. Cell number and transepithelial electrical resistance (TEER) reached steady state 7-8 days after inoculation on the Transwell and TEER values at steady state were 130-140 Ω cm2, which was higher or lower than that of small intestine or Caco-2 cells, respectively. The transport studies were carried out using the cultured epithelium on the Transwell. The transport of D-glucose across the cultured stratified layer of oral epithelial cells was much more extensive than L-glucose, and was inhibited by 2-deoxy-D-glucose, a substrate of facilitative glucose transporters, and α-methyl-D-glucoside, a specific substrate of a Na+/glucose cotransporter (SGLT1). The results indicate that the sugar transporters function not only to take up D-glucose by the epithelial cells but also to transport the sugar across the stratified epithelial layer.
UR - http://www.scopus.com/inward/record.url?scp=0036171524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036171524&partnerID=8YFLogxK
U2 - 10.1211/0022357021778402
DO - 10.1211/0022357021778402
M3 - Article
C2 - 11848285
AN - SCOPUS:0036171524
VL - 54
SP - 213
EP - 219
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
SN - 0022-3573
IS - 2
ER -