Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation

Rina Muraoka, Hidetsugu Tsujigiwa, Keisuke Nakano, Naoki Katase, Ryo Tamamura, Mihoko Tomida, Norimasa Okafuji, Hitoshi Nagatsuka, Toshiyuki Kawakami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We examined the transplanted bone marrow-derived cell migration into periodontal tissues. Bone marrow cells from green fluorescent protein (GFP) transgenic mice were transplanted. The immunohistochemistry (IHC) revealed that GFP-positive cells were detected in the periodontal tissues. The GFP-positive cells histopathologically differentiated into some cell types. The fluorescence IHC and TRAP staining techniques demonstrated these cells were detected as osteoclasts and macrophages. Furthermore, GFP-positive cells gathered adjacent blood vessels. The data suggest that GFP-positive bone marrow-derived cell migrate into periodontal tissues and differentiate periodontal tissue component-cells.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalJournal of Hard Tissue Biology
Volume20
Issue number4
DOIs
Publication statusPublished - 2011

Fingerprint

Green Fluorescent Proteins
Bone Marrow Cells
Cell Movement
Cell Differentiation
Bone
Tissue
Proteins
Cells
Immunohistochemistry
Macrophages
Blood vessels
Osteoclasts
Cellular Structures
Transgenic Mice
Blood Vessels
Fluorescence
Staining and Labeling

Keywords

  • Bone marrow-derived cells
  • Fibroblasts
  • Green fluorescent protein (GFP)
  • Macrophages
  • Osteoclasts
  • Periodontal tissues

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation. / Muraoka, Rina; Tsujigiwa, Hidetsugu; Nakano, Keisuke; Katase, Naoki; Tamamura, Ryo; Tomida, Mihoko; Okafuji, Norimasa; Nagatsuka, Hitoshi; Kawakami, Toshiyuki.

In: Journal of Hard Tissue Biology, Vol. 20, No. 4, 2011, p. 301-306.

Research output: Contribution to journalArticle

Muraoka, R, Tsujigiwa, H, Nakano, K, Katase, N, Tamamura, R, Tomida, M, Okafuji, N, Nagatsuka, H & Kawakami, T 2011, 'Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation', Journal of Hard Tissue Biology, vol. 20, no. 4, pp. 301-306. https://doi.org/10.2485/jhtb.20.301
Muraoka, Rina ; Tsujigiwa, Hidetsugu ; Nakano, Keisuke ; Katase, Naoki ; Tamamura, Ryo ; Tomida, Mihoko ; Okafuji, Norimasa ; Nagatsuka, Hitoshi ; Kawakami, Toshiyuki. / Transplanted bone marrow-derived cell migration into periodontal tissues and cell differentiation. In: Journal of Hard Tissue Biology. 2011 ; Vol. 20, No. 4. pp. 301-306.
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