Translational repression by the cis-acting element of structure-anchored repression (CAESAR) of human ctgf/ccn2 mRNA

Research output: Contribution to journalArticle

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Abstract

The cis-acting element of structure-anchored repression (CAESAR) is a post-transcriptional regulatory element of gene expression, which is located in the 3′-untranslated region (UTR) of the human ccn2 gene (ctgf/ccn2). In this report, the repression mechanism of CAESAR, as well as the structural requirement, was investigated. Removal of minor stem-loops from CAESAR resulted in proportional attenuation of the repressive function, whereas removal of the single bulge or modification of primary nucleotide sequence did not affect its functionality. In light of functional mechanism, CAESAR exerted no significant effects on stability or nuclear export of the cis-linked mRNA. However, this element significantly interfered with the association of such mRNA on ribosome and slowed down the translation process thereafter in vitro. A translation repression mechanism by RNA secondary structure to determine the basal ctgf/ccn2 expression level was uncovered herein.

Original languageEnglish
Pages (from-to)3751-3758
Number of pages8
JournalFEBS Letters
Volume579
Issue number17
DOIs
Publication statusPublished - Jul 27 2005

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Transcriptional Regulatory Elements
Messenger RNA
Cell Nucleus Active Transport
3' Untranslated Regions
Regulator Genes
Ribosomes
Gene expression
Nucleotides
Genes
RNA
Gene Expression
In Vitro Techniques

Keywords

  • ccn2
  • ctgf
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Translational repression by the cis-acting element of structure-anchored repression (CAESAR) of human ctgf/ccn2 mRNA. / Kubota, Satoshi; Mukudai, Yoshiki; Moritani, Norifumi; Nakao, Kyouji; Kawata, Kazumi; Takigawa, Masaharu.

In: FEBS Letters, Vol. 579, No. 17, 27.07.2005, p. 3751-3758.

Research output: Contribution to journalArticle

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