Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Hiroshi Kuroda, Haruka Suzuki, Takahiro Kusumegi, Tetsuro Hirose, Yasushi Yukawa, Masahiro Sugiura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

Original languageEnglish
Pages (from-to)1374-1378
Number of pages5
JournalPlant and Cell Physiology
Volume48
Issue number9
DOIs
Publication statusPublished - Sep 1 2007
Externally publishedYes

Keywords

  • GUG codon
  • In vitro
  • Shine-Dalgarno sequence
  • Tobacco
  • Translation
  • psbC

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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