Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

Hiroshi Kuroda, Haruka Suzuki, Takahiro Kusumegi, Tetsuro Hirose, Yasushi Yukawa, Masahiro Sugiura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

Original languageEnglish
Pages (from-to)1374-1378
Number of pages5
JournalPlant and Cell Physiology
Volume48
Issue number9
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

Fingerprint

Protein Biosynthesis
Chloroplasts
translation (genetics)
Tobacco
Plastids
tobacco
chloroplasts
Messenger RNA
plastids
Initiator Codon
Point Mutation
start codon
point mutation
Nucleotides
nucleotides
ribosomal RNA
Genes
organisms
genes

Keywords

  • GUG codon
  • In vitro
  • psbC
  • Shine-Dalgarno sequence
  • Tobacco
  • Translation

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts. / Kuroda, Hiroshi; Suzuki, Haruka; Kusumegi, Takahiro; Hirose, Tetsuro; Yukawa, Yasushi; Sugiura, Masahiro.

In: Plant and Cell Physiology, Vol. 48, No. 9, 09.2007, p. 1374-1378.

Research output: Contribution to journalArticle

Kuroda, Hiroshi ; Suzuki, Haruka ; Kusumegi, Takahiro ; Hirose, Tetsuro ; Yukawa, Yasushi ; Sugiura, Masahiro. / Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 9. pp. 1374-1378.
@article{9cb2e979c1fa4e269b1ad3e6ae4c6997,
title = "Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts",
abstract = "The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.",
keywords = "GUG codon, In vitro, psbC, Shine-Dalgarno sequence, Tobacco, Translation",
author = "Hiroshi Kuroda and Haruka Suzuki and Takahiro Kusumegi and Tetsuro Hirose and Yasushi Yukawa and Masahiro Sugiura",
year = "2007",
month = "9",
doi = "10.1093/pcp/pcm097",
language = "English",
volume = "48",
pages = "1374--1378",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Translation of psbC mRNAs starts from the downstream GUG, not the upstream AUG, and requires the extended Shine-Dalgarno sequence in tobacco chloroplasts

AU - Kuroda, Hiroshi

AU - Suzuki, Haruka

AU - Kusumegi, Takahiro

AU - Hirose, Tetsuro

AU - Yukawa, Yasushi

AU - Sugiura, Masahiro

PY - 2007/9

Y1 - 2007/9

N2 - The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

AB - The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine-Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3′ end of 16S rRNA facilitates translation initiation from the GUG.

KW - GUG codon

KW - In vitro

KW - psbC

KW - Shine-Dalgarno sequence

KW - Tobacco

KW - Translation

UR - http://www.scopus.com/inward/record.url?scp=34548808708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548808708&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcm097

DO - 10.1093/pcp/pcm097

M3 - Article

C2 - 17664183

AN - SCOPUS:34548808708

VL - 48

SP - 1374

EP - 1378

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 9

ER -