Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma

Kei Takemoto, Keiki Ogino, Masafumi Shibamori, Toshikazu Gondo, Yoshiaki Hitomi, Tomoko Takigawa, Da Hong Wang, Jiro Takaki, Hiroshi Ichimura, Yoshihisa Fujikura, Hironobu Ishiyama

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46 Citations (Scopus)

Abstract

Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 μg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.

Original languageEnglish
Pages (from-to)L1419-L1426
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume293
Issue number6
DOIs
Publication statusPublished - Dec 2007

Keywords

  • Airway remodeling
  • Mite
  • NC/Nga
  • Reactive nitrogen species

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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    Takemoto, K., Ogino, K., Shibamori, M., Gondo, T., Hitomi, Y., Takigawa, T., Wang, D. H., Takaki, J., Ichimura, H., Fujikura, Y., & Ishiyama, H. (2007). Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma. American Journal of Physiology - Lung Cellular and Molecular Physiology, 293(6), L1419-L1426. https://doi.org/10.1152/ajplung.00418.2006