Abstract
Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 μg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 293 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- Airway remodeling
- Mite
- NC/Nga
- Reactive nitrogen species
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Cell Biology
- Physiology
Cite this
Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma. / Takemoto, Kei; Ogino, Keiki; Shibamori, Masafumi; Gondo, Toshikazu; Hitomi, Yoshiaki; Takigawa, Tomoko; Wang, Da Hong; Takaki, Jiro; Ichimura, Hiroshi; Fujikura, Yoshihisa; Ishiyama, Hironobu.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 293, No. 6, 12.2007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transiently, paralleled upregulation of arginase and nitric oxide synthase and the effect of both enzymes on the pathology of asthma
AU - Takemoto, Kei
AU - Ogino, Keiki
AU - Shibamori, Masafumi
AU - Gondo, Toshikazu
AU - Hitomi, Yoshiaki
AU - Takigawa, Tomoko
AU - Wang, Da Hong
AU - Takaki, Jiro
AU - Ichimura, Hiroshi
AU - Fujikura, Yoshihisa
AU - Ishiyama, Hironobu
PY - 2007/12
Y1 - 2007/12
N2 - Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 μg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.
AB - Changes in the expression of arginase and their association with nitrosative stress were investigated using an asthmatic model previously established in NC/Nga mice with mite extract. Mite crude extract (100 μg/day) from Dermatophagoides farinae was administered intranasally for 5 consecutive days (day 0-4), and a single challenge was performed on day 11. On day 12, upregulation of the mRNA expression of inducible types of nitric oxide synthase (iNOS) and increases in immunohistochemical staining for iNOS and nitrotyrosine were observed. However, the level of nitrite + nitrate was unchanged. An increase in enzymatic activity, upregulation of mRNA expression, and immunostaining for arginase I was detected in the lung tissue and serum. Moreover, increases in both arginase I and II were revealed by immunoblotting. Goblet cell hyperplasia in bronchial epithelial cells and increasing collagen synthesis around the bronchus were also observed. These results suggested that an increase in arginase may lead to decreased availability of arginine for nitric oxide synthase and may contribute to the remodeling of the lung.
KW - Airway remodeling
KW - Mite
KW - NC/Nga
KW - Reactive nitrogen species
UR - http://www.scopus.com/inward/record.url?scp=37149036630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37149036630&partnerID=8YFLogxK
U2 - 10.1152/ajplung.00418.2006
DO - 10.1152/ajplung.00418.2006
M3 - Article
VL - 293
JO - American Journal of Physiology - Lung Cellular and Molecular Physiology
JF - American Journal of Physiology - Lung Cellular and Molecular Physiology
SN - 1040-0605
IS - 6
ER -