Transient increase of cytokines in the acute ischemic tissue is beneficial to cell-based therapeutic angiogenesis

Shu Lan Qin, Tao Sheng Li, Masayuki Kubo, Mako Ohshima, Akira Furutani, Kimikazu Hamano

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Implantation of bone marrow cells (BMCs) is a treatment of ischemic disease. It is well known that many inflammatory cytokines are released in ischemic tissue, especially in the acute phase, so in the present study it was investigated if the transient increase of cytokines in the acute ischemic tissue influences cell-based therapeutic angiogenesis. Methods and Results: Ischemic limb models were created in C57BL/6 mice as 24 h (acute) or 2 weeks (chronic) after ischemia. BMCs were cultured with total tissue protein, which was extracted from the acute and chronic ischemic muscles. The survival, adhesion, and migration of BMCs were significantly better after culture with 1 mg/ml total tissue protein extracted from the acute ischemic limbs than from the chronic ischemic limbs (p<0.001). For the in-vivo study, 8×10 6 BMCs, collected from green fluorescent protein (GFP) transgenic mice, were implanted into the acute or chronic ischemic limbs of the mice. The survival of implanted cells and blood flow were significantly better when BMCs were implanted into the acute ischemic limbs than into the chronic ischemic limbs (p<0.001). Conclusions: A transient increase of cytokines in the acute ischemic tissue is beneficial for cell-based therapeutic angiogenesis.

Original languageEnglish
Pages (from-to)2075-2080
Number of pages6
JournalCirculation Journal
Volume72
Issue number12
DOIs
Publication statusPublished - Dec 3 2008

Keywords

  • Acute ischemic tissue
  • Angiogenesis
  • Bone marrow cells
  • Cytokines

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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