Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

Tadaaki Hashimoto; Tetsuji Yamada; Akiko Tada; Shinji Kawamata; Yoshikazu Tanaka; Pernpong Sriprasertsak; Yuki Ichinose; Hisaharu Kato; Satoshi Izutsu; Tomonori Shiraishi; Hachiro Oku; Yoshiaki Ohtsuki

doi:10.1007/BF00232529

Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

Tadaaki Hashimoto, Tetsuji Yamada, Akiko Tada, Shinji Kawamata, Yoshikazu Tanaka, Pernpong Sriprasertsak, Yuki Ichinose, Hisaharu Kato, Satoshi Izutsu, Tomonori Shiraishi, Hachiro Oku, Yoshiaki Ohtsuki

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

Original language	English
Pages (from-to)	183-187
Number of pages	5
Journal	Plant Cell Reports
Volume	11
Issue number	4
DOIs	https://doi.org/10.1007/BF00232529
Publication status	Published - May 1 1992

Keywords

Phenylalanine ammonia-lyase
Pisum sativum L. Midoriusui
Protoplast
Suspension culture
Transient assay

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/BF00232529

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title = "Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter",

abstract = "High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.",

keywords = "Phenylalanine ammonia-lyase, Pisum sativum L. Midoriusui, Protoplast, Suspension culture, Transient assay",

author = "Tadaaki Hashimoto and Tetsuji Yamada and Akiko Tada and Shinji Kawamata and Yoshikazu Tanaka and Pernpong Sriprasertsak and Yuki Ichinose and Hisaharu Kato and Satoshi Izutsu and Tomonori Shiraishi and Hachiro Oku and Yoshiaki Ohtsuki",

year = "1992",

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doi = "10.1007/BF00232529",

language = "English",

volume = "11",

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T1 - Transient expression in electroporated pea protoplasts

T2 - Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

AU - Hashimoto, Tadaaki

AU - Yamada, Tetsuji

AU - Tada, Akiko

AU - Kawamata, Shinji

AU - Tanaka, Yoshikazu

AU - Sriprasertsak, Pernpong

AU - Ichinose, Yuki

AU - Kato, Hisaharu

AU - Izutsu, Satoshi

AU - Shiraishi, Tomonori

AU - Oku, Hachiro

AU - Ohtsuki, Yoshiaki

PY - 1992/5/1

Y1 - 1992/5/1

N2 - High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

AB - High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

KW - Phenylalanine ammonia-lyase

KW - Pisum sativum L. Midoriusui

KW - Protoplast

KW - Suspension culture

KW - Transient assay

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JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 4

ER -

Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

Abstract

Keywords

ASJC Scopus subject areas

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