Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

Tadaaki Hashimoto, Tetsuji Yamada, Akiko Tada, Shinji Kawamata, Yoshikazu Tanaka, Pernpong Sriprasertsak, Yuki Ichinose, Hisaharu Kato, Satoshi Izutsu, Tomonori Shiraishi, Hachiro Oku, Yoshiaki Ohtsuki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

High yields of viable pea protoplasts were produced from suspension cultured cells and the conditions for the optimum transient expression of the chloramphenicol acetyltransferase (CAT) gene fused to the CaMV 35S promoter after electroporation were investigated. Conditions for elicitor induction of a member of the phenylalanine ammonia-lyase (PAL) gene family in pea was also investigated using a chimeric gene carrying 480 bp of the putative promoter region of gPAL1 connected to bacterial cat gene and nos terminator. CAT activity was considerably induced by the treatment with fungal elicitor (>100 μg/ml glucose equivalent) isolated from Mycosphaerella pinodes, a pea pathogen.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalPlant Cell Reports
Volume11
Issue number4
DOIs
Publication statusPublished - May 1992

Fingerprint

phenylalanine ammonia-lyase
protoplasts
peas
promoter regions
chloramphenicol acetyltransferase
genes
Mycosphaerella pinodes
electroporation
cultured cells
cats
glucose
elicitors
pathogens

Keywords

  • Phenylalanine ammonia-lyase
  • Pisum sativum L. Midoriusui
  • Protoplast
  • Suspension culture
  • Transient assay

ASJC Scopus subject areas

  • Plant Science

Cite this

Hashimoto, T., Yamada, T., Tada, A., Kawamata, S., Tanaka, Y., Sriprasertsak, P., ... Ohtsuki, Y. (1992). Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter. Plant Cell Reports, 11(4), 183-187. https://doi.org/10.1007/BF00232529

Transient expression in electroporated pea protoplasts : Elicitor responsiveness of a phenylalanine ammonia-lyase promoter. / Hashimoto, Tadaaki; Yamada, Tetsuji; Tada, Akiko; Kawamata, Shinji; Tanaka, Yoshikazu; Sriprasertsak, Pernpong; Ichinose, Yuki; Kato, Hisaharu; Izutsu, Satoshi; Shiraishi, Tomonori; Oku, Hachiro; Ohtsuki, Yoshiaki.

In: Plant Cell Reports, Vol. 11, No. 4, 05.1992, p. 183-187.

Research output: Contribution to journalArticle

Hashimoto, T, Yamada, T, Tada, A, Kawamata, S, Tanaka, Y, Sriprasertsak, P, Ichinose, Y, Kato, H, Izutsu, S, Shiraishi, T, Oku, H & Ohtsuki, Y 1992, 'Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter', Plant Cell Reports, vol. 11, no. 4, pp. 183-187. https://doi.org/10.1007/BF00232529
Hashimoto, Tadaaki ; Yamada, Tetsuji ; Tada, Akiko ; Kawamata, Shinji ; Tanaka, Yoshikazu ; Sriprasertsak, Pernpong ; Ichinose, Yuki ; Kato, Hisaharu ; Izutsu, Satoshi ; Shiraishi, Tomonori ; Oku, Hachiro ; Ohtsuki, Yoshiaki. / Transient expression in electroporated pea protoplasts : Elicitor responsiveness of a phenylalanine ammonia-lyase promoter. In: Plant Cell Reports. 1992 ; Vol. 11, No. 4. pp. 183-187.
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