Transglucosylation activities of multiple forms of α-glucosidase from spinach

Manabu Sugimoto, Satoshi Furui, Kenji Sasaki, Yukio Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Transglucosylation activities of spinach α-glucosidase I and IV, which have different substrate specificity for hydrolyzing activity, were investigated. In a maltose mixture, α-glucosidase I, which has high activity toward not only maltooligosaccharides but also soluble starch and can hydrolyze isomaltose, produced maltotriose, isomaltose, and panose, and α-glucosidase IV, which has high activity toward maltooligosaccharides but faint activity toward soluble starch and isomaltose, produced maltotriose, kojibiose, and 2,4-di-α-D-glucosyl-glucose. Transglucosylation to sucrose by α-glucosidase I and IV resulted in the production of theanderose and erlose, respectively, showing that spinach α-glucosidase I and IV are useful to synthesize the α-1,6-glucosylated and α-1,2- and 1,4-glucosylated products, respectively.

Original languageEnglish
Pages (from-to)1160-1163
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number5
Publication statusPublished - May 2003

Fingerprint

Glucosidases
glucosidases
Isomaltose
Spinacia oleracea
spinach
isomaltose
maltotriose
Starch
2,4-Dichlorophenoxyacetic Acid
Maltose
starch
Substrate Specificity
Sucrose
Sugar (sucrose)
maltose
substrate specificity
Glucose
glucosidase I
sucrose
Substrates

Keywords

  • α-glucosidase
  • Spinach
  • Transglucosylation

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Transglucosylation activities of multiple forms of α-glucosidase from spinach. / Sugimoto, Manabu; Furui, Satoshi; Sasaki, Kenji; Suzuki, Yukio.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 5, 05.2003, p. 1160-1163.

Research output: Contribution to journalArticle

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