Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing

Hiroshi Hisano, Akira Kanazawa, Akira Kawakami, Midori Yoshida, Yoshiya Shimamoto, Toshihiko Yamada

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The accumulation of fructan in grasses during autumn is linked to winter hardiness. Genetic manipulation of the accumulation of fructan could be an important molecular breeding strategy for the improvement of winter hardiness in grasses. We produced transgenic perennial ryegrass (Lolium perenne) plants that overexpress wheat fructosyltransferase genes, wft1 and wft2, which encode sucrose-fructan 6-fructosyltransferase (6-SFT) and sucrose-sucrose 1-fructosyltransferase (1-SST), respectively, under the control of CaMV 35S promoter using a particle bombardment-mediated method of transformation. Significant increases in fructan content were detected in the transgenic perennial ryegrass plants. A freezing test using the electrical conductivity method indicated that transgenic plants that accumulated a greater amount of fructan than non-transgenic plants have increased tolerance on a cellular level to freezing. The results suggest that the overexpression of the genes involved in fructan synthesis serves as a novel strategy to produce freezing-tolerant grasses.

Original languageEnglish
Pages (from-to)861-868
Number of pages8
JournalPlant Science
Volume167
Issue number4
DOIs
Publication statusPublished - Oct 2004
Externally publishedYes

Fingerprint

inulosucrase
Fructans
Lolium
fructans
Lolium perenne
Freezing
Triticum
freezing
Genes
genetically modified organisms
Poaceae
wheat
winter hardiness
genes
sucrose
grasses
DNA Shuffling
Electric Conductivity
Genetically Modified Plants
gene overexpression

Keywords

  • 1-FFT
  • 2,4-D
  • 2,4-dichlorophenoxyacetic acid
  • 6-SFT
  • 6G-FFT
  • BAP
  • benzylaminopurine
  • CaMV
  • cauliflower mosaic virus
  • fructan-fructan 1-fructosyltransferase
  • fructan-fructan 6G-fructosyltransferase
  • high performance liquid chromatography
  • HPLC

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. / Hisano, Hiroshi; Kanazawa, Akira; Kawakami, Akira; Yoshida, Midori; Shimamoto, Yoshiya; Yamada, Toshihiko.

In: Plant Science, Vol. 167, No. 4, 10.2004, p. 861-868.

Research output: Contribution to journalArticle

Hisano, Hiroshi ; Kanazawa, Akira ; Kawakami, Akira ; Yoshida, Midori ; Shimamoto, Yoshiya ; Yamada, Toshihiko. / Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. In: Plant Science. 2004 ; Vol. 167, No. 4. pp. 861-868.
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