Transforming growth factor-α stimulates proliferation of mammotrophs and corticotrophs in the mouse pituitary

S. Oomizu, J. Honda, Sakae Takeuchi, T. Kakeya, T. Masui, S. Takahashi

Research output: Contribution to journalArticle

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Abstract

Oestrogen stimulates the proliferation of pituitary cells. The present study was designed to clarify the involvement of transforming growth factor- α (TGF-α) in the oestrogen-induced growth of mouse pituitary cells in vitro. Anterior pituitary cells obtained from ICR male mice were cultured in a primary serum-free culture system. Proliferation of pituitary cells was detected by monitoring the cellular uptake of bromodeoxyuridine. Secretory cell types were immunocytochemically determined. Treatment with TGF-α (0.1 and 1 ng/ml) for 5 days stimulated cell proliferation. Since TGF-α binds to the epidermal growth factor (EGF) receptor, this action may be exerted through the EGF receptor. Oestradiol-17β (OE2, 10-9 M) stimulated mammotrophic and corticotrophic cell proliferation. RG-13022, an EGF receptor inhibitor, inhibited the cell proliferation induced by EGF or OE2, showing that the EGF receptor was involved in the growth response in mammotrophs and corticotrophs. Treatment with antisense TGF-α oligodeoxynucleotide (ODN) inhibited the cell proliferation induced by OE2, but treatment with antisense EGF ODN did not. RT-PCR analysis revealed that OE2 stimulated TGF- α mRNA and EGF receptor mRNA expression. These results indicate that TGF-α mediates the stimulatory effect of oestrogen on the pituitary cell proliferation in a paracrine or autocrine manner, and that EGF receptor expression is stimulated by oestrogen.

Original languageEnglish
Pages (from-to)493-501
Number of pages9
JournalJournal of Endocrinology
Volume165
Issue number2
Publication statusPublished - 2000

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Corticotrophs
Transforming Growth Factors
Epidermal Growth Factor Receptor
Cell Proliferation
Estrogens
Oligodeoxyribonucleotides
Epidermal Growth Factor
Inbred ICR Mouse
Messenger RNA
Bromodeoxyuridine
Growth
Estradiol
Polymerase Chain Reaction
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

Transforming growth factor-α stimulates proliferation of mammotrophs and corticotrophs in the mouse pituitary. / Oomizu, S.; Honda, J.; Takeuchi, Sakae; Kakeya, T.; Masui, T.; Takahashi, S.

In: Journal of Endocrinology, Vol. 165, No. 2, 2000, p. 493-501.

Research output: Contribution to journalArticle

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AU - Masui, T.

AU - Takahashi, S.

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