Transforming growth factor-α mRNA expression and its possible roles in mouse endometrial stromal cells

Tetsuya Maekawa, Atsuko Sakuma, Shusuke Taniuchi, Yuki Ogo, Taisen Iguchi, Sakae Takeuchi, Sumio Takahashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transforming growth factor-α (TGFα) is thought to be involved in the regulation of endometrial cells. We investigated Tgfa mRNA expression, and the effects of TGFα on DNA-synthesis and gene expression of insulin-like growth factor 1 (IGF1), IGF binding protein-3 (IGFBP3) and IGF1 receptor in the mouse endometrial cells, because IGF1 is involved in estrogen-induced growth of endometrial cells. We also investigated the role of TGF on matrix metalloproteinase (MMP) expression, as MMPs are involved both in tissue remodeling during cell proliferation and in enhancement of IGF1 signaling through the degradation of IGFBP3. Tgfa mRNA expression was detected in endometrial luminal and glandular epithelial cells, and stromal cells. Tgfa mRNA signals did not appear to change in endometrial luminal epithelial cells, but signals in glandular epithelial cells were higher at diestrus 1, 2 and proestrus, and the number of stromal cells showing strong signals appeared to increase at diestrus 1 and 2. Endometrial epithelial and stromal cells were treated with estradiol-17β (E2) or progesterone (P4). E2 or P4 stimulated Tgfa mRNA expression in stromal cells. TGFα stimulated DNA synthesis in endometrial epithelial and stromal cells, while E2 and P4 stimulated DNA synthesis in stromal cells. In stromal cells, TGFα, at as low as 1 ng/ml, decreased Igfbp3 and Mmp9 mRNA levels, while high dose (10 ng/ml) of TGFα decreased Igf1 mRNA level and increased Mmp3 mRNA level. These results imply that TGFα stimulates proliferation of endometrial stromal cells through multiple mechanisms, including its regulation of Igfbp3 and Mmp3 transcription.

Original languageEnglish
Pages (from-to)377-383
Number of pages7
JournalZoological Science
Volume29
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

transforming growth factors
stromal cells
mice
epithelial cells
somatomedins
diestrus
insulin-like growth factor binding proteins
synthesis
DNA
proestrus
metalloproteinases
estrogens
estradiol
cell growth
progesterone
cell proliferation
transcription (genetics)
cells
gene expression
degradation

Keywords

  • endometrium
  • mouse
  • reproduction
  • TGFα
  • uterus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Transforming growth factor-α mRNA expression and its possible roles in mouse endometrial stromal cells. / Maekawa, Tetsuya; Sakuma, Atsuko; Taniuchi, Shusuke; Ogo, Yuki; Iguchi, Taisen; Takeuchi, Sakae; Takahashi, Sumio.

In: Zoological Science, Vol. 29, No. 6, 06.2012, p. 377-383.

Research output: Contribution to journalArticle

Maekawa, Tetsuya ; Sakuma, Atsuko ; Taniuchi, Shusuke ; Ogo, Yuki ; Iguchi, Taisen ; Takeuchi, Sakae ; Takahashi, Sumio. / Transforming growth factor-α mRNA expression and its possible roles in mouse endometrial stromal cells. In: Zoological Science. 2012 ; Vol. 29, No. 6. pp. 377-383.
@article{eb61892645eb476287d10f013aae65bc,
title = "Transforming growth factor-α mRNA expression and its possible roles in mouse endometrial stromal cells",
abstract = "Transforming growth factor-α (TGFα) is thought to be involved in the regulation of endometrial cells. We investigated Tgfa mRNA expression, and the effects of TGFα on DNA-synthesis and gene expression of insulin-like growth factor 1 (IGF1), IGF binding protein-3 (IGFBP3) and IGF1 receptor in the mouse endometrial cells, because IGF1 is involved in estrogen-induced growth of endometrial cells. We also investigated the role of TGF on matrix metalloproteinase (MMP) expression, as MMPs are involved both in tissue remodeling during cell proliferation and in enhancement of IGF1 signaling through the degradation of IGFBP3. Tgfa mRNA expression was detected in endometrial luminal and glandular epithelial cells, and stromal cells. Tgfa mRNA signals did not appear to change in endometrial luminal epithelial cells, but signals in glandular epithelial cells were higher at diestrus 1, 2 and proestrus, and the number of stromal cells showing strong signals appeared to increase at diestrus 1 and 2. Endometrial epithelial and stromal cells were treated with estradiol-17β (E2) or progesterone (P4). E2 or P4 stimulated Tgfa mRNA expression in stromal cells. TGFα stimulated DNA synthesis in endometrial epithelial and stromal cells, while E2 and P4 stimulated DNA synthesis in stromal cells. In stromal cells, TGFα, at as low as 1 ng/ml, decreased Igfbp3 and Mmp9 mRNA levels, while high dose (10 ng/ml) of TGFα decreased Igf1 mRNA level and increased Mmp3 mRNA level. These results imply that TGFα stimulates proliferation of endometrial stromal cells through multiple mechanisms, including its regulation of Igfbp3 and Mmp3 transcription.",
keywords = "endometrium, mouse, reproduction, TGFα, uterus",
author = "Tetsuya Maekawa and Atsuko Sakuma and Shusuke Taniuchi and Yuki Ogo and Taisen Iguchi and Sakae Takeuchi and Sumio Takahashi",
year = "2012",
month = "6",
doi = "10.2108/zsj.29.377",
language = "English",
volume = "29",
pages = "377--383",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "6",

}

TY - JOUR

T1 - Transforming growth factor-α mRNA expression and its possible roles in mouse endometrial stromal cells

AU - Maekawa, Tetsuya

AU - Sakuma, Atsuko

AU - Taniuchi, Shusuke

AU - Ogo, Yuki

AU - Iguchi, Taisen

AU - Takeuchi, Sakae

AU - Takahashi, Sumio

PY - 2012/6

Y1 - 2012/6

N2 - Transforming growth factor-α (TGFα) is thought to be involved in the regulation of endometrial cells. We investigated Tgfa mRNA expression, and the effects of TGFα on DNA-synthesis and gene expression of insulin-like growth factor 1 (IGF1), IGF binding protein-3 (IGFBP3) and IGF1 receptor in the mouse endometrial cells, because IGF1 is involved in estrogen-induced growth of endometrial cells. We also investigated the role of TGF on matrix metalloproteinase (MMP) expression, as MMPs are involved both in tissue remodeling during cell proliferation and in enhancement of IGF1 signaling through the degradation of IGFBP3. Tgfa mRNA expression was detected in endometrial luminal and glandular epithelial cells, and stromal cells. Tgfa mRNA signals did not appear to change in endometrial luminal epithelial cells, but signals in glandular epithelial cells were higher at diestrus 1, 2 and proestrus, and the number of stromal cells showing strong signals appeared to increase at diestrus 1 and 2. Endometrial epithelial and stromal cells were treated with estradiol-17β (E2) or progesterone (P4). E2 or P4 stimulated Tgfa mRNA expression in stromal cells. TGFα stimulated DNA synthesis in endometrial epithelial and stromal cells, while E2 and P4 stimulated DNA synthesis in stromal cells. In stromal cells, TGFα, at as low as 1 ng/ml, decreased Igfbp3 and Mmp9 mRNA levels, while high dose (10 ng/ml) of TGFα decreased Igf1 mRNA level and increased Mmp3 mRNA level. These results imply that TGFα stimulates proliferation of endometrial stromal cells through multiple mechanisms, including its regulation of Igfbp3 and Mmp3 transcription.

AB - Transforming growth factor-α (TGFα) is thought to be involved in the regulation of endometrial cells. We investigated Tgfa mRNA expression, and the effects of TGFα on DNA-synthesis and gene expression of insulin-like growth factor 1 (IGF1), IGF binding protein-3 (IGFBP3) and IGF1 receptor in the mouse endometrial cells, because IGF1 is involved in estrogen-induced growth of endometrial cells. We also investigated the role of TGF on matrix metalloproteinase (MMP) expression, as MMPs are involved both in tissue remodeling during cell proliferation and in enhancement of IGF1 signaling through the degradation of IGFBP3. Tgfa mRNA expression was detected in endometrial luminal and glandular epithelial cells, and stromal cells. Tgfa mRNA signals did not appear to change in endometrial luminal epithelial cells, but signals in glandular epithelial cells were higher at diestrus 1, 2 and proestrus, and the number of stromal cells showing strong signals appeared to increase at diestrus 1 and 2. Endometrial epithelial and stromal cells were treated with estradiol-17β (E2) or progesterone (P4). E2 or P4 stimulated Tgfa mRNA expression in stromal cells. TGFα stimulated DNA synthesis in endometrial epithelial and stromal cells, while E2 and P4 stimulated DNA synthesis in stromal cells. In stromal cells, TGFα, at as low as 1 ng/ml, decreased Igfbp3 and Mmp9 mRNA levels, while high dose (10 ng/ml) of TGFα decreased Igf1 mRNA level and increased Mmp3 mRNA level. These results imply that TGFα stimulates proliferation of endometrial stromal cells through multiple mechanisms, including its regulation of Igfbp3 and Mmp3 transcription.

KW - endometrium

KW - mouse

KW - reproduction

KW - TGFα

KW - uterus

UR - http://www.scopus.com/inward/record.url?scp=84861756031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861756031&partnerID=8YFLogxK

U2 - 10.2108/zsj.29.377

DO - 10.2108/zsj.29.377

M3 - Article

C2 - 22639808

AN - SCOPUS:84861756031

VL - 29

SP - 377

EP - 383

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 6

ER -