Transformation of the Acidophilic Heterotroph Acidiphilium facilis by Electroporation

Kenji Inagaki, Tatsuo Tano, Hidehiko Tanaka, Noriaki Kishimoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We constructed a cloning vector for use in the acidophilic heterotroph Acidiphilium facilis. The vector pAH1O1 (8.8kb) was constructed from a 6.1 kb restriction fragment of the Acidiphilium plasmid pAU1 and a pUC19 carrying a β-lactamase gene. The antibiotic resistance gene was efficiently expressed in A. facilis. Several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 × 103 transformants per μg of plasmid DNA at a field strength of 10kV/cm with a 7.0ms pulse.

Original languageEnglish
Pages (from-to)1770-1771
Number of pages2
JournalBioscience, Biotechnology, and Biochemistry
Volume57
Issue number10
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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